Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus

Kluyveromyces marxianus GX-15 was mutated multiple times by alternately treatment with UV irradiation and NTG for two cycles. Four mutant strains with improved ethanol yield were obtained. The maximum ethanol concentration, ethanol yield coefficient and theoretical ethanol yield of the best mutant s...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2010-12, Vol.32 (12), p.1847-1851
Main Authors: Pang, Zong-wen, Liang, Jing-juan, Qin, Xiao-juan, Wang, Ji-rui, Feng, Jia-xun, Huang, Ri-bo
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Coefficients
Ethanol
Ethanol - metabolism
Ethanol fermentation
Ethyl alcohol
Fermentation
Fundamental and applied biological sciences. Psychology
Glucose
Glucose - metabolism
Irradiation
Kluyveromyces - drug effects
Kluyveromyces - genetics
Kluyveromyces - metabolism
Kluyveromyces - radiation effects
Kluyveromyces marxianus
Life Sciences
Metabolic Networks and Pathways - genetics
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microbiology
Molecular Sequence Data
Mutagenesis
Mutagens - metabolism
Mutation
Nitrosoguanidines - metabolism
Original Research Paper
Sequence Analysis, DNA
Strain
Ultraviolet Rays
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Kluyveromyces marxianus GX-15 was mutated multiple times by alternately treatment with UV irradiation and NTG for two cycles. Four mutant strains with improved ethanol yield were obtained. The maximum ethanol concentration, ethanol yield coefficient and theoretical ethanol yield of the best mutant strain, GX-UN120, was 69 g/l, 0.46 g/g and 91%, respectively, when fermenting 150 g glucose/l at 40°C. The corresponding values for GX-15 were 58 g/l, 0.39 g/g and 76%, respectively. GX-UN120 grew well in 11% (v/v) of ethanol, while GX-15 could not grow when ethanol was greater than 8% (v/v).
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-010-0384-8