Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for characterization of Culicoides nubeculosus biting midges

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has shown promise in species identification of insect species. We evaluated its potential to consistently characterize laboratory-reared biting midges of the species Culicoides nubeculosus (Meigen) (Diptera:...

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Bibliographic Details
Published in:Medical and veterinary entomology 2011-03, Vol.25 (1), p.32-38
Main Authors: KAUFMANN, C, ZIEGLER, D, SCHAFFNER, F, CARPENTER, S, PFLÜGER, V, MATHIS, A
Format: Article
Language:English
Subjects:
Aedes
Aedes - chemistry
Aedes - classification
Aging
Animals
Biomarkers - analysis
Biomarkers - chemistry
biting midge
Ceratopogonidae
Ceratopogonidae - chemistry
Ceratopogonidae - classification
Cluster Analysis
Culicidae
Culicoides
Culicoides nubeculosus
Desorption
Diptera
Feeding Behavior
Female
Forcipomyia
Insect Proteins - analysis
Insect Proteins - chemistry
Ions
Lasers
MALDI-TOF MS
Male
Mass spectrometry
mosquito
Preservation, Biological
Principal Component Analysis
Proteomics - methods
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has shown promise in species identification of insect species. We evaluated its potential to consistently characterize laboratory-reared biting midges of the species Culicoides nubeculosus (Meigen) (Diptera: Ceratopogonidae). Twenty-one reproducible potential biomarker masses for C. nubeculosus were identified under different experimental treatments. These treatments included the homogenization of insects in either water or known concentrations of formic acid. The biomarker masses were present independent of age, gender and different periods of storage of individuals in 70% ethanol (a standard preservation method). It was found that the presence of blood in females reduced the intensity of the MALDI-TOF pattern, necessitating the removal of the abdomen before analysis. The protein profiles of a related non-biting midge, Forcipomyia sp. (Diptera: Ceratopogonidae), and of Aedes japonicus japonicus (Theobald) (Diptera: Culicidae) mosquitoes were also examined and were distinctly different. These findings provide preliminary data to optimize future studies in differentiation of species within the Culicoides genus using MALDI-TOF MS which is a rapid, simple, reliable and cost-effective technique.
ISSN:0269-283X
1365-2915
DOI:10.1111/j.1365-2915.2010.00927.x