Resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in rolC transgenic cell cultures of Vitis amurensis

Resveratrol, a naturally occurring polyphenol, has been reported to exhibit a wide range of valuable biological and pharmacological properties. In the present investigation, we show that transformation of Vitis amurensis Rupr. with the oncogene rolC of Agrobacterium rhizogenes increased resveratrol...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2010-10, Vol.88 (3), p.727-736
Main Authors: Dubrovina, Alexandra S., Manyakhin, Artem Y., Zhuravlev, Yuri N., Kiselev, Konstantin V.
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
Acyltransferases - metabolism
Agrobacterium rhizogenes
Ammonia
Analysis
Applied Genetics and Molecular Biotechnology
Bacteria
Biomedical and Life Sciences
Biotechnology
Cell culture
Cells, Cultured
Gene expression
Gene Expression Regulation, Plant
Genes, Bacterial
Genomes
Life Sciences
Metabolism
Microbial Genetics and Genomics
Microbiology
Multigene Family
Pharmaceuticals
Phenylalanine Ammonia-Lyase - genetics
Phenylalanine Ammonia-Lyase - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
Proteins
Rhizobium - genetics
RNA, Plant - genetics
Soil sciences
Stilbenes - metabolism
Studies
Vectors (Biology)
Vitis
Vitis - enzymology
Vitis - genetics
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Summary:Resveratrol, a naturally occurring polyphenol, has been reported to exhibit a wide range of valuable biological and pharmacological properties. In the present investigation, we show that transformation of Vitis amurensis Rupr. with the oncogene rolC of Agrobacterium rhizogenes increased resveratrol production in the two transformed callus cultures 3.7 and 11.9 times. The rolC -transformed calli were capable of producing 0.099% and 0.144% dry weight of resveratrol. We characterized phenylalanine ammonia-lyase ( PAL ) and stilbene synthase ( STS ) gene expression in the two rolC transgenic callus cultures of V. amurensis . In the rolC transgenic culture with higher resveratrol content, expression of VaPAL3 , VaSTS3 , VaSTS4 , VaSTS5 , VaSTS6 , VaSTS8 , VaSTS9 , and VaSTS10 was increased; while in the rolC culture with lower resveratrol content, expression of VaPAL3 and VaSTS9 was increased. We suggest that transformation of V. amurensis calli with the rolŠ” gene induced resveratrol accumulation via selective enhancement of expression of individual PAL and STS genes involved in resveratrol biosynthesis. We compared the data on PAL and STS gene expression in rolC transgenic calli with the previously obtained results for rolB transgenic calli of V. amurensis . We propose that the transformation of V. amurensis with the rolC and rolB genes of A. rhizogenes increased resveratrol accumulation through different regulatory pathways.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-010-2792-z