mab21l2 transgenics reveal novel expression patterns of mab21l1 and mab21l2, and conserved promoter regulation without sequence conservation

mab21l1 and mab21l2 paralogs have widespread and dynamic expression patterns during vertebrate development. Both genes are expressed in the developing eye, midbrain, neural tube, and branchial arches. Our goal was to identify promoter regions with activity in mab21l2 expression domains. Assays of ma...

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Bibliographic Details
Published in:Developmental dynamics 2011-04, Vol.240 (4), p.745-754
Main Authors: Cederlund, Maria L., Vendrell, Victor, Morrissey, Maria E., Yin, Jun, Gaora, Peadar Ó., Smyth, Vincent A., Higgins, Desmond G., Kennedy, Breandán N.
Format: Article
Language:English
Subjects:
amacrine
Amacrine Cells - metabolism
Amacrine Cells - physiology
Animals
Animals, Genetically Modified
Base Sequence
Conserved Sequence
Embryo, Nonmammalian
Eye - embryology
Eye - metabolism
eye, retina
Gene Expression Regulation, Developmental
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Homeodomain Proteins - physiology
mab21l1
mab21l2
Molecular Sequence Data
promoter
Promoter Regions, Genetic
Sequence Homology, Nucleic Acid
Tissue Distribution
Transgenes
zebrafish
Zebrafish - genetics
Zebrafish - metabolism
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
Zebrafish Proteins - physiology
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Summary:mab21l1 and mab21l2 paralogs have widespread and dynamic expression patterns during vertebrate development. Both genes are expressed in the developing eye, midbrain, neural tube, and branchial arches. Our goal was to identify promoter regions with activity in mab21l2 expression domains. Assays of mab21l2 promoter‐EGFP constructs in zebrafish embryos confirm that constructs containing 7.2 or 4.9 kb of mab21l2 promoter region are sufficient to drive expression in known (e.g., tectum, branchial arches) and unexpected domains (e.g., lens and retinal amacrine cells). A comparative analysis identifies complementary and novel expression domains of endogenous mab21l2 (e.g., lens and ventral iridocorneal canal) and mab21l1 (e.g., retinal amacrine and ganglion cells). Interestingly, therefore, despite the absence of conserved non‐coding elements, a 4.9‐kb mab21l2 promoter is sufficient to recapitulate expression in tissues unique to mab21l1 or mab21l2. Developmental Dynamics 240:745–754, 2011. © 2011 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.22573