A transgenic wnt8a:PAC reporter reveals biphasic regulation of vertebrate mesoderm development

Vertebrate wnt8a links anteroposterior and dorsoventral axis patterning, but the regulation of wnt8a expression and its relationship to mesoderm induction and maintenance pathways is unclear. To address this, we have generated zebrafish transgenic for a modified genomic PAC clone that expresses EGFP...

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Bibliographic Details
Published in:Developmental dynamics 2011-04, Vol.240 (4), p.898-907
Main Authors: Narayanan, Anand, Thompson, Sarah A., Lee, Jared J., Lekven, Arne C.
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Body Patterning - genetics
Body Patterning - physiology
Brachyury
Chromosomes, Artificial, P1 Bacteriophage - genetics
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
Embryo, Nonmammalian
Gene Expression Regulation, Developmental - physiology
Gene Regulatory Networks - physiology
Genes, Reporter - physiology
Mesoderm - embryology
Mesoderm - metabolism
Models, Biological
Nodal
posterior mesoderm
Stem Cells - metabolism
Stem Cells - physiology
Tail - embryology
Tail - metabolism
ventrolateral mesoderm
vertebrate
Vertebrates - embryology
Vertebrates - genetics
Vertebrates - metabolism
Wnt Proteins - genetics
Wnt Proteins - metabolism
wnt8a
zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Summary:Vertebrate wnt8a links anteroposterior and dorsoventral axis patterning, but the regulation of wnt8a expression and its relationship to mesoderm induction and maintenance pathways is unclear. To address this, we have generated zebrafish transgenic for a modified genomic PAC clone that expresses EGFP from the wnt8a locus. The EGFP reporter transgene is expressed in a pattern nearly identical to wnt8a, including maternal deposition, expression in the ventrolateral mesoderm and in the yolk syncytial layer. Loss of function studies show that wnt8a expression is under biphasic control by Nodal and No Tail/Brachyury, whereby early phase expression is Nodal‐dependent but late phase expression is Ntl/Bra dependent. EGFP fluorescence persists in cells that transcribe the reporter, thus comprising a tracer for ventrolaterally derived mesodermal lineages. We use this property to show that wnt8a expression marks Nodal‐independent tail mesoderm formation and that Ntl/Bra predominantly regulates wnt8a in paraxial mesoderm progenitors. Developmental Dynamics 240:898–907, 2011. © 2011 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.22599