Ex vivo expansion of mesenchymal stromal cells

Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical appl...

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Bibliographic Details
Published in:Best practice & research. Clinical haematology 2011-03, Vol.24 (1), p.73-81
Main Authors: Bernardo, Maria Ester, MD, PhD, Cometa, Angela Maria, PhD, Pagliara, Daria, MD, Vinti, Luciana, MD, Rossi, Francesca, MD, Cristantielli, Rosaria, PhD, Palumbo, Giuseppe, MD, PhD, Locatelli, Franco, MD, PhD
Format: Article
Language:English
Subjects:
Bone Marrow Cells
Cell Culture Techniques - methods
Cell Proliferation
Cell Separation
Cells, Cultured
cellular therapy
Culture Media, Serum-Free
ex vivo expansion
fetal calf serum
Hematology, Oncology and Palliative Medicine
Humans
malignant transformation
mesenchymal stromal cells
Mesenchymal Stromal Cells - cytology
Multipotent Stem Cells
platelet lysate
Stromal Cells - cytology
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Summary:Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed.
ISSN:1521-6926
1532-1924
DOI:10.1016/j.beha.2010.11.002