Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleac...

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Bibliographic Details
Published in:Physiological and molecular plant pathology 2010-12, Vol.75 (1), p.13-22
Main Authors: Idris, Ali M., Tuttle, J.R., Robertson, D., Haigler, C.H., Brown, J.K.
Format: Article
Language:English
Subjects:
Begomovirus
Biological and medical sciences
Cotton breeding
Cotton genetics
Cotton leaf crumple virus
Fundamental and applied biological sciences. Psychology
Geminivirus
Genetic improvement
Genetics of eukaryotes. Biological and molecular evolution
Gossypium hirsutum
Magnesium chelatase marker
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Viral episome
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Summary:A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. ► Biolistic inoculation of Cotton leaf crumple virus-VIGS resulted in systemic and persistent photo-bleaching in seven cotton cultivars. ► The intensity of silencing was variable. ► Acala SJ-1 was identified as an optimal candidate for CLCrV-VIGS-based cotton reverse-genetics.
ISSN:0885-5765
1096-1178
DOI:10.1016/j.pmpp.2010.07.002