Cryopreservation of human failed maturation oocytes shows that vitrification gives superior outcomes to slow cooling

This study investigated whether failed maturation oocytes could be used to evaluate different cryopreservation procedures. A total of 289 failed maturation oocytes (GV and MI stages), obtained from 169 patients undergoing IVF treatment (mean age 33.84 ± 5.0) were divided into two different slow-cool...

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Bibliographic Details
Published in:Cryobiology 2010-12, Vol.61 (3), p.243-247
Main Authors: Fasano, G., Vannin, A.S., Biramane, J., Delbaere, A., Englert, Y.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Birth control
Cell Survival
Choline - pharmacology
Cryopreservation - methods
Diverse techniques
Female
Fertilization and development rates
Fundamental and applied biological sciences. Psychology
Gynecology. Andrology. Obstetrics
Human oocyte cryopreservation
Humans
In vitro maturation rate
Medical sciences
Molecular and cellular biology
Oocytes - drug effects
Oocytes - physiology
Slow-cooling
Sodium Chloride - pharmacology
Sterility. Assisted procreation
Survival rate
Vitrification
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Summary:This study investigated whether failed maturation oocytes could be used to evaluate different cryopreservation procedures. A total of 289 failed maturation oocytes (GV and MI stages), obtained from 169 patients undergoing IVF treatment (mean age 33.84 ± 5.0) were divided into two different slow-cooling groups (1.5 mol/l 1,2-propanediol + 0.2 mol/l sucrose in either NaCl (group A) or choline chloride (ChCl) (group B) based cryopreservation solutions) and one vitrification group (15% ethylene glycol + 15% dimethyl sulphoxide). Survival rate, in vitro maturation (IVM) rate, fertilization and developmental rate of cryopreserved oocytes were assessed. Regardless of the stage at which cryopreservation was performed (GV + MI), the slow cooling with ChCl based medium always gave significantly lower survival rate than the slow cooling in NaCl based medium ( p = 0.01) and vitrification ( p < 0.001). An extended study also showed statistically reduced survival rate between slow-cooling NaCl based medium and vitrification ( p < 0.05). Global results of in vitro maturation and fertilization showed worse results between both slow-cooling NaCl and ChCl based media versus vitrification. In conclusion, for oocytes that had failed to mature, vitrification gave better survival, maturation, fertilization and also cleavage rates than the slow-cooling protocols. Four cells embryos were obtained only from vitrified in vitro matured MI oocytes.
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2010.08.002