ARDesigner: A web-based system for allosteric RNA design

RNA molecules play vital informational, structural, and functional roles in molecular biology, making them ideal targets for synthetic biology. However, several challenges remain for engineering novel allosteric RNA molecules, and the development of efficient computational design techniques is vital...

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Bibliographic Details
Published in:Journal of biotechnology 2010-12, Vol.150 (4), p.466-473
Main Authors: Shu, Wenjie, Liu, Ming, Chen, Hebing, Bo, Xiaochen, Wang, Shengqi
Format: Article
Language:English
Subjects:
Allosteric Regulation - genetics
Allosteric RNA
Biological and medical sciences
Biotechnology
Computational Biology - methods
Fundamental and applied biological sciences. Psychology
Genetic Engineering - methods
Internet
Mutational robustness
RNA - chemistry
RNA design
RNA synthetic biology
Software
Temperature
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Summary:RNA molecules play vital informational, structural, and functional roles in molecular biology, making them ideal targets for synthetic biology. However, several challenges remain for engineering novel allosteric RNA molecules, and the development of efficient computational design techniques is vitally needed. Here we describe the development of Allosteric RNA Designer (ARDesigner), a user-friendly and freely available web-based system for allosteric RNA design that incorporates mutational robustness in the design process. The system output includes detailed design information in a graphical HTML format. We used ARDesigner to engineer a temperature-sensitive AR, and found that the resulting design satisfied the prescribed properties/input. ARDesigner provides a simple means for researchers to design allosteric RNAs with specific properties. With its versatile framework and possibilities for further enhancement, ARDesigner may serve as a useful tool for synthetic biologists and therapeutic design. ARDesigner and its executable version are freely available at http://biotech.bmi.ac.cn/ARDesigner.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2010.10.067