Evaluation of quality control strategies in Scutellaria herbal medicines

The statutory regulation of herbal medicines is under review within the United Kingdom (UK) and by 2011 all herbal medicines will require either a Product Licence or a Traditional Herbal Registration. The species Scutellaria baicalensis has been shown to possess anti-inflammatory, anti-viral and ant...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2011-04, Vol.54 (5), p.951-957
Main Authors: Boyle, Susanne P., Doolan, Paul J., Andrews, Clare E., Reid, Raymond G.
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
antioxidant activity
Antioxidant coefficient
baicalin
Biological and medical sciences
biomarkers
Chromatographic fingerprint
Chromatography, High Pressure Liquid
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - isolation & purification
Drugs, Chinese Herbal - standards
free radical scavengers
Fundamental and applied biological sciences. Psychology
General pharmacology
Government Regulation
Herbal Medicine - legislation & jurisprudence
Herbal Medicine - standards
Herbal medicines
high performance liquid chromatography
Legislation, Drug
Medical sciences
Molecular Structure
multivariate analysis
Pharmacology. Drug treatments
phenols
Plant Extracts - analysis
Plant Extracts - isolation & purification
Plant Extracts - standards
Quality Control
Scutellaria
Scutellaria baicalensis
United Kingdom
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Summary:The statutory regulation of herbal medicines is under review within the United Kingdom (UK) and by 2011 all herbal medicines will require either a Product Licence or a Traditional Herbal Registration. The species Scutellaria baicalensis has been shown to possess anti-inflammatory, anti-viral and anti-tumor properties and is one of the most widely used Chinese herbal extracts in Eastern and Western medicines. The bioactivity of this herbal medicine is due to the radical scavenging activities of the flavone components of which there are more than 60. This research has characterised 5 key flavones in 18 extracts of Scutellaria using a combination of HPLC with DAD and MS detection. Employing an internal standard approach, the validated HPLC method afforded good sensitivity and excellent assay precision. Assays for the ferric reducing antioxidant power (FRAP) and total phenol determinations enabled determination of the antioxidant coefficient (PAC) of each Scutellaria extract. The potential usefulness of employing multivariate statistical analysis using a combination of the key parameters collected namely, FRAP activity, total phenol content, levels of 5 flavone biomarkers and the PAC as a means of quality evaluation of the Scutellaria herbal extracts was investigated. The PAC value was predicted by soft independent modelling of class analogy (SIMCA) as being the most discriminatory parameter and applying this ranking the herbal extracts were grouped into 3 clusters. The second most influential parameter in determining the clustering of the samples was the level of baicalin in each extract. It is proposed that the PAC value alone or in combination with a chromatographic fingerprint of key biomarkers [e.g. baicalin or (baicalin + baicalein)] may be useful indicators to adopt for the quality control of S. baicalensis.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2010.11.019