Crystallization of Cytochrome c-553 in Aerobically Grown Pseudomonas denitrificans

Cytochrome c-553 was crystallized from the soluble fraction of aerobically grown cells of Pseudomonas denitrificans. Its molecular weight was estimated to be 45,000. It had two haem prosthetic groups per molecule; one of them showed reduced α-peak at about 551 mμ and the other at about 555 mμ. Their...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1969-12, Vol.66 (6), p.775-781
Main Authors: IWASAKI, HIDEKAZU, SHIDARA, SOHSUKE
Format: Article
Language:English
Subjects:
Azides
Carbon Monoxide
Chromatography, DEAE-Cellulose
Chromatography, Gel
Crystallization
Cyanides
Cytochromes - isolation & purification
Hydrogen-Ion Concentration
Iron
Molecular Weight
Oxidation-Reduction
Pseudomonas - analysis
Spectrophotometry
Ultracentrifugation
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Summary:Cytochrome c-553 was crystallized from the soluble fraction of aerobically grown cells of Pseudomonas denitrificans. Its molecular weight was estimated to be 45,000. It had two haem prosthetic groups per molecule; one of them showed reduced α-peak at about 551 mμ and the other at about 555 mμ. Their E0′ (pH7) values were estimated to be −0.09 V and between 0 and −0.09 V, respectively. The cytochrome was readily autooxidizable. The ferro-form reacted with CO, and the ferro-and ferri-forms reacted with KCN at pH 7.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a129207