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Metabolism of Histones of Brain and Liver
Turnover of whole histones of brain and liver nuclei was studied after administration of 14 C-lysine to adult mice. The radioactivity of histones and other nuclear and cytoplasmic proteins was determined at intervals from 2 hours to 245 days. Two fractions of cerebral histones with halflifetimes of...
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Published in: | The Journal of biological chemistry 1966-05, Vol.241 (10), p.2397-2404 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Turnover of whole histones of brain and liver nuclei was studied after administration of 14 C-lysine to adult mice. The radioactivity of histones and other nuclear and cytoplasmic proteins was determined at intervals
from 2 hours to 245 days. Two fractions of cerebral histones with halflifetimes of 54 and 104 days and three fractions of
hepatic histones with half-lifetimes of 18, 56, and 93 days were found. A larger number of fractions with gradually increasing
turnover rates are not excluded.
The histones of brain and liver turn over at a lower rate than any of the nuclear and cytoplasmic protein fractions measured
in the respective organs.
In order to label histones with a very low rate of turnover, 14 C-lysine was administered to pregnant mice and the turnover of histones was estimated in their offspring after reaching maturity.
In the brain only a slow histone fraction with a half-lifetime of 117 days and in the liver two fractions with half-lifetimes
of 58 and 105 days were observed.
Over a period from 2 to 8½ months in the brain and from 5 to 8½ months in the liver the decrease in the specific activity
of histones was very small, corresponding to a replacement rate of about 0.6% per day. The turnover rates of histones may
reflect division and replacement of various cellular species. The data suggest a metabolic stability of histones which corresponds
to that of deoxyribonucleic acid. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(18)96633-0 |