Murine Colon Adenocarcinoma: Syngeneic Orthotopic Transplantation and Subsequent Hepatic Metastases

Syngeneic murine colon adenocarcinoma (MCA-38) cells were transplanted in the submucosa of distal colon, proximal colon, cecum, ileum, jejunum, and duodenum of male C57BL/6 mice, with local lymphoid follicles used as points of entry. The tumor grew best at the cecum and led to liver and mesenteric l...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1977-11, Vol.59 (5), p.1537-1544
Main Authors: Tan, M. H., Holyoke, E. D., Goldrosen, M. H.
Format: Article
Language:English
Subjects:
Adenocarcinoma - pathology
Animals
Cecal Neoplasms - pathology
Colonic Neoplasms - pathology
Duodenal Neoplasms - pathology
Ileum
Intestinal Neoplasms - pathology
Jejunum
Liver Neoplasms - pathology
Lymphatic Metastasis
Male
Mice
Mice, Inbred C57BL
Neoplasm Metastasis
Neoplasm Transplantation
Neutrophils - pathology
Transplantation, Isogeneic
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Summary:Syngeneic murine colon adenocarcinoma (MCA-38) cells were transplanted in the submucosa of distal colon, proximal colon, cecum, ileum, jejunum, and duodenum of male C57BL/6 mice, with local lymphoid follicles used as points of entry. The tumor grew best at the cecum and led to liver and mesenteric lymph node metastases in 8 and 9 weeks, respectively, after transplantation. Histologically, a local inflammatory reaction involving polymorphonuclear leukocytes was observed within 48–72 hours following transplantation; after this time, the microscopic tumor foci began to grow progressively. Mononuclear lymphoid cells of the gut-associated lymphoid tissue did not infiltrate the progressively growing tumor; however, polymor-phonuclear leukocytes were constantly observed at the tumor periphery in the lamina propria. The studies indicated that orthotopic transplantation as a model system can provide a means of examining the role of the local immune response as a focus of host resistance and as a factor in metastatic tumor spread. The findings also suggested the usefulness of this model in immunotherapeutic and chemotherapeutic studies of secondary hepatic disease.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/59.5.1537