Supercoiled DNA of nuclear polyhedrosis virus of Galleria mellonella L

Supercoiled infectious DNA was isolated from nuclear polyhedrosis virus infecting great wax moth Galleria mellonella L.) Covalently closed DNA molecules constitute approximately 10--30 per cent in our preparations. These molecules dissappear during storage. Electron micrographs of supercoiled and op...

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Bibliographic Details
Published in:Archives of virology 1977-01, Vol.53 (1/2), p.79-86
Main Authors: Skuratovskaya, I.N, Strokovskaya, L.I, Zherebtsova, E.N, Gudz-Gorban, A.P
Format: Article
Language:English
Subjects:
Animals
arthropods
DNA, Circular - analysis
DNA, Viral - analysis
entomology
Hot Temperature
Insect Viruses - analysis
Insect Viruses - pathogenicity
insects
Lepidoptera - microbiology
Microscopy, Electron
Molecular Weight
Moths - microbiology
Nucleic Acid Denaturation
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Summary:Supercoiled infectious DNA was isolated from nuclear polyhedrosis virus infecting great wax moth Galleria mellonella L.) Covalently closed DNA molecules constitute approximately 10--30 per cent in our preparations. These molecules dissappear during storage. Electron micrographs of supercoiled and open circular molecules are presented. Length of the open rings is about 50--52 micron. Infectivity of different DNA forms is discussed.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01314849