Bacterial Adherence to Virus-Infected Cells: A Cell Culture Model of Bacterial Superinfection

MDCK cells (a line of stable canine kidney cells) infected with influenza A/NWS/33 virus (a neurotropic variant of the Wilson Smith strain) were tested with 18 selected bacterial species to determine whether mammalian cells become susceptible to bacterial adherence as a result of virus infection. Ce...

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Bibliographic Details
Published in:The Journal of infectious diseases 1978-02, Vol.137 (2), p.176-181
Main Authors: Sanford, Barbara A., Shelokov, Alexis, Ramsay, Mary A.
Format: Article
Language:English
Subjects:
Adhesiveness
Bacteria
Bacterial adhesion
Bacterial Physiological Phenomena
Cell culture techniques
Cell Transformation, Viral
Cells, Cultured
Hemadsorption
Hemadsorption Inhibition Tests
Infections
Influenza A virus
Kidney cells
Major Articles
Orthomyxoviridae
Streptococcus
Streptococcus agalactiae
Viruses
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Summary:MDCK cells (a line of stable canine kidney cells) infected with influenza A/NWS/33 virus (a neurotropic variant of the Wilson Smith strain) were tested with 18 selected bacterial species to determine whether mammalian cells become susceptible to bacterial adherence as a result of virus infection. Cell monolayers were washed and examined microscopically for adherence. Bacteria of only two of 18 species were seen to adhere to the infected cells: a group B Streptococcus and Streptococcus sanguis. Control monolayers were negative for adherence. Pretreatment of virus-infected cultures with mouse ascitic fluid containing antibody to influenza A virus completely blocked adherence of the bacteria. Further testing with the strains representative of the five serotypes of group B Streptococcus disclosed that adherence occurred with types Ia, Ic, and II, but not with types Ib and III.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/137.2.176