Characterization of Human Anti-Factor VIII Antibodies Purified by Immune Complex Formation

Anti–factor VIII antibodies from three hemophilia A patients were selectively isolated by immune complex formation and secondary precipitation with polyethylene glycol. These antibodies were characterized as IgG, with only one type of light chains (kappa) and a mixture of IgG3 and IgG4 heavy chains....

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Bibliographic Details
Published in:Blood 1976-12, Vol.48 (6), p.931-939
Main Authors: Lavergne, J.M., Meyer, Dominique, Reisner, H.
Format: Article
Language:English
Subjects:
Antibodies - analysis
Antibodies - isolation & purification
Antigen-Antibody Complex
Factor VIII - immunology
Humans
Immunoglobulin G - analysis
Immunoglobulin Light Chains - analysis
Polyethylene Glycols - pharmacology
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Summary:Anti–factor VIII antibodies from three hemophilia A patients were selectively isolated by immune complex formation and secondary precipitation with polyethylene glycol. These antibodies were characterized as IgG, with only one type of light chains (kappa) and a mixture of IgG3 and IgG4 heavy chains. This finding indicated that stable complexes of factor VIII and homologous anti–factor VIII antibodies could be obtained, and that the immunologic response to the infusion of homologous factor VIII was oligoclonal rather than monoclonal in these patients.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V48.6.931.931