Clec14a is specifically expressed in endothelial cells and mediates cell to cell adhesion

► Clec14a is specifically expressed in various kinds of endothelial cells (ECs) and vasculature of mice. ► Clec14a mediates endothelial cell–cell adhesion via C-type lectin domain. ► Knockdown of Clec14a in ECs resulted in suppression of endothelial cell migration and tube formation on Matrigel. Cle...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2011-01, Vol.404 (1), p.103-108
Main Authors: Rho, Seung-Sik, Choi, Hyun-Jung, Min, Jeong-Ki, Lee, Heon-Woo, Park, Hongryeol, Park, Hyojin, Kim, Young-Myeong, Kwon, Young-Guen
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
C-type lectin like domain
Cell Adhesion
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - physiology
cell movement
Cells, Cultured
Cell–cell adhesion
Clec14a
Endothelial cell
endothelial cells
Endothelial Cells - physiology
Endothelium, Vascular - cytology
Endothelium, Vascular - physiology
Female
Gene Deletion
Gene Silencing
Humans
Lectins, C-Type - genetics
Lectins, C-Type - physiology
membrane proteins
Membrane Proteins - genetics
Membrane Proteins - physiology
Mice
Mice, Inbred C57BL
mutants
Neovascularization, Physiologic
plasma membrane
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Summary:► Clec14a is specifically expressed in various kinds of endothelial cells (ECs) and vasculature of mice. ► Clec14a mediates endothelial cell–cell adhesion via C-type lectin domain. ► Knockdown of Clec14a in ECs resulted in suppression of endothelial cell migration and tube formation on Matrigel. Clec14a is a member of the thrombomodulin (TM) family, but its function has not yet been determined. Here, we report that Clec14a is a plasma membrane protein of endothelial cells (ECs) expressed specifically in the vasculature of mice. Deletion mutant analysis revealed that Clec14a mediates cell–cell adhesion through its C-type lectin-like domain. Knockdown of Clec14a in ECs suppressed cell migratory activity and filopodial protrusion, and delayed formation of tube-like structures. These findings demonstrate that Clec14a is a novel EC-specific protein that appears to play a role in cell–cell adhesion and angiogenesis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2010.11.075