Replication of Reticuloendotheliosis Viruses in Cell Culture: Chronic Infection

McArdle Laboratory, University of Wisconsin, Madison, Wisconsin 53706, U.S.A. After an initial acute infection with cell killing, chicken or duck embryo fibroblasts infected in culture with reticuloendotheliosis viruses set up a chronic infection with no cell killing or morphological transformation....

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Bibliographic Details
Published in:Journal of general virology 1975-06, Vol.27 (3), p.267-274
Main Authors: Temin, H. M, Kassner, Victoria K
Format: Article
Language:English
Subjects:
Alpharetrovirus - growth & development
Animals
Birds
Cell Line
Chick Embryo
Clone Cells
Cytarabine - pharmacology
Cytopathogenic Effect, Viral
Ducks
Fibroblasts
Mitomycins - pharmacology
Newcastle disease virus - growth & development
Reticuloendotheliosis virus - growth & development
Retroviridae - growth & development
Time Factors
Vesicular stomatitis Indiana virus - growth & development
Viral Interference
Viral Plaque Assay
Virus Replication - drug effects
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Summary:McArdle Laboratory, University of Wisconsin, Madison, Wisconsin 53706, U.S.A. After an initial acute infection with cell killing, chicken or duck embryo fibroblasts infected in culture with reticuloendotheliosis viruses set up a chronic infection with no cell killing or morphological transformation. Essentially all of the chronically infected cells produced virus. The virus production was not sensitive to cytosine arabinoside or mitomycin C as was virus production in an acute infection. The chronically infected cells had a strong group-specific resistance to the c.p.e. of superinfecting reticuloendotheliosis viruses. However, they were sensitive to vesicular stomatitis virus and avian leukosis-sarcoma viruses. After double infection, single cells produced reticuloendotheliosis virus and avian leukosis-sarcoma virus. Received 15 October 1974; accepted 22 January 1975.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-27-3-267