A comparison of glycosyltransferase activities and malignant properties in normal and transformed cells derived from BALB/c mice

The ability of suspensions of BALB/c cells to catalyze the incorporation of nucleotide sugars into complex polysaccharides has been compared. These cells have previously been characterized for concanavalin A-induced agglutinability, tumorigenicity, and malignancy. All of the cell lines tested cataly...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1975-02, Vol.35 (2), p.438-441
Main Authors: Patt, L M, Van Nest, G A, Grimes, W J
Format: Article
Language:English
Subjects:
Agglutination
Animals
Cell Transformation, Neoplastic
Cells, Cultured - enzymology
Concanavalin A - pharmacology
Cytosine
Galactosamine
Galactose
Glucosamine
Glycolipids - biosynthesis
Glycoproteins - biosynthesis
Glycosides
Guanidines
Hexosyltransferases - metabolism
Mannose
Mice
Mice, Inbred BALB C
Neoplasms, Experimental - etiology
Neuraminic Acids
Nucleoside Diphosphate Sugars - metabolism
Nucleotides
Uridine Diphosphate Sugars - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The ability of suspensions of BALB/c cells to catalyze the incorporation of nucleotide sugars into complex polysaccharides has been compared. These cells have previously been characterized for concanavalin A-induced agglutinability, tumorigenicity, and malignancy. All of the cell lines tested catalyze transfer of the sugar moieties of cytosine 5-monophosphate N-acetylneuraminic acid, uridine 5-diphosphate galactose, uridine 5-diphosphate N-acetylgalactosamine, uridine 5-diphosphate N-acetylglucosamine, uridine 5-diphosphate glucose, and guanidine 5-diphosphate monnose into glycoproteins and glycolipids. While some transformed lines exhibit alterations in transferase levels, others cannot be distinguished from normal cells. Normal cells, transformed cells that cause tumors that regress, and transformed cells that cause tumors that kill an immunologically competent host show growth-dependent changes in transferase activities. Determining the ability to catalyze carbohydrate transfer is insufficient for predicting the tumorigenic and malignant properties of a cell line.
ISSN:0008-5472
1538-7445