Large scale enzymatic synthesis of nucleoside-5′-monophosphates using a phosphotransferase from carrots

The enzymatic synthesis of nucleoside‐5′‐monophosphates from purineriboside, 6‐mercapto‐purine riboside, 6‐methylmercapto‐purine riboside, 6‐chloro‐purine riboside, tubercidin, 8‐aza‐adenosine, and 3′‐deoxy‐adenosine is described in gram scale. The synthesis is catalyzed by a phosphotransferase from...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1974-11, Vol.16 (11), p.1449-1458
Main Authors: Marutzky, R., Peterssen-Borstel, H., Flossdorf, J., Kula, M.-R.
Format: Article
Language:English
Subjects:
Adenine - metabolism
Aza Compounds - metabolism
Chromatography, DEAE-Cellulose
Chromatography, Gel
Deoxyadenosines - metabolism
Mercaptopurine - metabolism
Nitrophenols - metabolism
Nucleosides - biosynthesis
Nucleotides - biosynthesis
Nucleotides - isolation & purification
Oxidative Phosphorylation
Phosphates - biosynthesis
Phosphates - metabolism
Phosphoric Monoester Hydrolases - analysis
Phosphotransferases - analysis
Phosphotransferases - isolation & purification
Phosphotransferases - metabolism
Plants, Edible - enzymology
Purine Nucleosides - metabolism
Pyrimidines - metabolism
Pyrroles - metabolism
Ribonucleosides - metabolism
Vegetables
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Summary:The enzymatic synthesis of nucleoside‐5′‐monophosphates from purineriboside, 6‐mercapto‐purine riboside, 6‐methylmercapto‐purine riboside, 6‐chloro‐purine riboside, tubercidin, 8‐aza‐adenosine, and 3′‐deoxy‐adenosine is described in gram scale. The synthesis is catalyzed by a phosphotransferase from carrots and uses phenylphosphate as phosphate donor. The reaction products are purified on QAE‐Sephadex A25 columns. The large scale preparation of the enzyme is also reported.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260161103