Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1

The protein elicitor from the mycelium of Alternaria tenuissima has been isolated. The elicitor triggered resistance to the tobacco mosaic virus in tobacco by inducing relative oxygen species, but without causing hypersensitive necrosis. The elicitor is reported to impart resistance against Verticil...

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Bibliographic Details
Published in:Science China. Life sciences 2010-12, Vol.53 (12), p.1410-1417
Main Authors: Li, GuangYue, Yang, XiuFen, Zeng, HongMei, Mao, JianJun, Liu, Hua, Zhang, YunHua, Qiu, DeWen
Format: Article
Language:English
Subjects:
Alternaria - chemistry
Alternaria - pathogenicity
Animals
Arabidopsis - chemistry
Arabidopsis - microbiology
Arabidopsis Proteins - analysis
Biomedical and Life Sciences
Fungal Proteins - analysis
Isotope Labeling - methods
Life Sciences
Mass Spectrometry - methods
Mycoses - microbiology
Plant Diseases - microbiology
Research Papers
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Summary:The protein elicitor from the mycelium of Alternaria tenuissima has been isolated. The elicitor triggered resistance to the tobacco mosaic virus in tobacco by inducing relative oxygen species, but without causing hypersensitive necrosis. The elicitor is reported to impart resistance against Verticillium dahliae and to increase yield in cotton, but its mechanism is not yet clear. In this study, the stable isotope labelled mass spectrometry method was used to quantify the relative abundances of protein expression induced by PeaT1 in Arabidopsis . A significant difference in the relative abundances for the expression of different proteins related to metabolism, modification, regulatory, defense, stress and antioxidation was found in Arabidopsis .
ISSN:1674-7305
1869-1889
DOI:10.1007/s11427-010-4091-4