Refining hot-water extracted silk sericin by ethanol-induced precipitation

In order to improve some inherent disadvantages in the mechanical properties of silk sericin (SS), we prepared ethanol-precipitated sericin (EpSS) by adding ethanol into hot-water extracted sericin (HS) solution. EpSS had higher viscosity compared to HS and it was due to the differences in their mol...

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Bibliographic Details
Published in:International journal of biological macromolecules 2011, Vol.48 (1), p.32-37
Main Authors: Oh, Hanjin, Lee, Ji Young, Kim, Moo Kon, Um, In Chul, Lee, Ki Hoon
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Animals
Biomechanical Phenomena
Bombyx - chemistry
Bombyx mori
Calorimetry, Differential Scanning
Chemical Precipitation
Circular Dichroism
Compressive Strength
Ethanol - chemistry
Hot Temperature
Hydrophobic and Hydrophilic Interactions
Molecular Weight
Protein Structure, Secondary
Protein-based material
Sericin
Sericins - chemistry
Sericins - isolation & purification
Silk
Solvents - chemistry
Viscosity
Water - chemistry
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Summary:In order to improve some inherent disadvantages in the mechanical properties of silk sericin (SS), we prepared ethanol-precipitated sericin (EpSS) by adding ethanol into hot-water extracted sericin (HS) solution. EpSS had higher viscosity compared to HS and it was due to the differences in their molecular weight distribution (MWD). The different MWD of EpSS was due to the different solubility of sericin molecules at various concentrations of ethanol, and the amino acid composition of EpSS showed that the more hydrophobic sericin is precipitated more readily in the presence of ethanol. The secondary structure of sericin is also changed from a random coil to β-sheet structure when the amount of ethanol added is high enough. The DSC analysis also revealed that EpSSs has a more compact structure. Finally, when beads were prepared from EpSS, they had enhanced compressive strength compared to those from HS.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2010.09.008