Pro-inflammatory cytokine and chemokine mRNA blood level in multiple sclerosis is related to treatment response and interferon-beta dose

Abstract Of 37 multiple sclerosis patients, 19 suboptimal responders were randomized to 375 ( n = 12) or 250 µg ( n = 7) interferon (IFN)-β-1b. mRNA levels of 23 cytokines, chemokines, and chemokine receptors were quantified by TaqMan® low-density array (TLDA) real-time polymerase chain reaction. Be...

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Published in:Journal of neuroimmunology 2010-09, Vol.226 (1), p.150-157
Main Authors: Cucci, Angele, Barbero, Pierangelo, Clerico, Marinella, Ferrero, Bruno, Versino, Elisabetta, Contessa, Giulia, Demercanti, Stefania, Viglietta, Emanuela, Di Liberto, Alessandra, Vai, Alessandra Giai, Durelli, Luca
Format: Article
Language:English
Subjects:
Adult
Allergy and Immunology
Blood levels
Chemokines
Cytokines
Cytokines - blood
Cytokines - classification
Cytokines - genetics
Dose-Response Relationship, Drug
Female
Gene Expression Regulation - drug effects
Humans
Interferon-beta - pharmacology
Interferon-beta - therapeutic use
Interferon-β (IFN-β) treatment response indicators
Longitudinal Studies
Male
mRNA
Multiple sclerosis
Multiple Sclerosis - drug therapy
Multiple Sclerosis - immunology
Neurology
RNA, Messenger - blood
Young Adult
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Summary:Abstract Of 37 multiple sclerosis patients, 19 suboptimal responders were randomized to 375 ( n = 12) or 250 µg ( n = 7) interferon (IFN)-β-1b. mRNA levels of 23 cytokines, chemokines, and chemokine receptors were quantified by TaqMan® low-density array (TLDA) real-time polymerase chain reaction. Better treatment responses or increased IFN-β doses were associated with elevated IL-10 and TGF-β and decreased CXCL10, IL-18, IFN-γ, and TNF-α transcript levels. Adjusting for dose, poor treatment responses resulted in a 4-fold increase in CXCL10 and IFN-γ expression (Mantel-Haenszel RR = 3.74, p < 0.0001). CXCL10 and IFN-γ mRNA levels were reliable indicators of treatment response. TLDA can be used to tailor IFN-β-1b therapy.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2010.05.038