The direct effects of GLP-1 and GIP, alone or in combination, on human pancreatic islets

GLP-1 and GIP are incretins known to affect beta-cell function and turnover. However, information on the direct actions of these hormones on human islet cells is limited. We tested the effects of acute (45 min) or prolonged (2 days) exposure to GLP-1 or GIP, alone or in combination, on the function...

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Bibliographic Details
Published in:Regulatory peptides 2010-12, Vol.165 (2), p.129-132
Main Authors: Lupi, R., Del Guerra, S., D'Aleo, V., Boggi, U., Filipponi, F., Marchetti, P.
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biological and medical sciences
Diabetes. Impaired glucose tolerance
Drug Combinations
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Female
Fundamental and applied biological sciences. Psychology
Gastric Inhibitory Polypeptide - pharmacology
GIP
GLP-1
Glucagon-Like Peptide 1 - pharmacology
Humans
Incretins
Incretins - pharmacology
Insulin - metabolism
Insulin Secretion
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Male
Medical sciences
Middle Aged
Organ Culture Techniques
Pancreatic islets
Reverse Transcriptase Polymerase Chain Reaction
Type 2 diabetes
Vertebrates: endocrinology
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Summary:GLP-1 and GIP are incretins known to affect beta-cell function and turnover. However, information on the direct actions of these hormones on human islet cells is limited. We tested the effects of acute (45 min) or prolonged (2 days) exposure to GLP-1 or GIP, alone or in combination, on the function and some molecular features of human islets isolated from non-diabetic and type 2 diabetic multiorgan donors. Acutely, both GLP-1 and, more markedly so, GIP, significantly potentiated glucose-stimulated insulin release, with no apparent synergic action. Some of these effects were observed with type 2 diabetic islets as well. Following prolonged exposure to the incretins, improved insulin secretion was observed, and transcription of insulin, PDX-1 and Bcl-2 was increased in both non-diabetic and diabetic islets, with the combination of GLP-1 and GIP showing more significant effects. Although it is still unclear at what extent these beta-cell direct actions of individual or combined incretins occur in-vivo in humans, nevertheless the results of the present study suggest that enhancing the exposure of pancreatic islets to circulating levels of both incretins may be useful for therapeutical purposes.
ISSN:0167-0115
1873-1686
DOI:10.1016/j.regpep.2010.04.009