Dibutyryl c-AMP as an inducer of sporidia formation: biochemical and antigenic changes during morphological differentiation of Karnal bunt (Tilletia indica) pathogen in axenic culture

Effect of dibutyryl adenosine 3',5'-cyclic monophosphate (dbc-AMP), an analogue of c-AMP, was investigated on growth and morphological differentiation of Tilletia indica. Exponential growth was observed up to 21 days in both presence and absence of dbc-AMP; however, increasing concentratio...

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Bibliographic Details
Published in:Journal of biosciences 2004-03, Vol.29 (1), p.23-31
Main Authors: Kumar, Anil, Tripathi, Kaushlendra, Rana, Manish, Purwar, Shalini, Garg, G R
Format: Article
Language:English
Subjects:
Antigens, Fungal - metabolism
Basidiomycota - drug effects
Basidiomycota - growth & development
Basidiomycota - metabolism
Bucladesine - pharmacology
Culture Media
Enzyme-Linked Immunosorbent Assay
Fungal Proteins - metabolism
Proteins
Reproduction, Asexual - drug effects
Signal Transduction - drug effects
Tilletia indica
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Summary:Effect of dibutyryl adenosine 3',5'-cyclic monophosphate (dbc-AMP), an analogue of c-AMP, was investigated on growth and morphological differentiation of Tilletia indica. Exponential growth was observed up to 21 days in both presence and absence of dbc-AMP; however, increasing concentration of dbc-AMP was deleterious to mycelial growth in liquid culture. A slow increase of mycelial biomass up to 21 days and decline at 30 days in the presence of 2.5 mM dbc-AMP was observed, therefore, this concentration was chosen in subsequent investigations. The inhibitory influence of dbc-AMP was further substantiated by decrease in soluble protein. The fungus on exposure to dbc-AMP experienced morphological differentiation from vegetative mycelial phase to sporogenous mycelial phase, and was induced to produce filiform sporidia. Use of quantitative ELISA further suggested that sporidia formation took more than 21 days in the presence of dbc-AMP. Variations of proteins during different stages of T. indica grown in the presence and absence of dbc-AMP suggested the expression of stage-specific proteins or differential expression of proteins induced by dbc-AMP. The changes in expression of cell surface antigens as evidenced from decrease and increase binding of anti-mycelial and anti-sporidial antibodies in dbc-AMP treated culture by ELISA was further interpreted on the basis of morphological differentiation from mycelial to sporidial phase
ISSN:0250-5991
0973-7138
DOI:10.1007/BF02702558