Simultaneous saccharification and cofermentation of dilute-acid pretreated yellow poplar hardwood to ethanol using xylose-fermenting Zymomonas mobilis

Simultaneous saccharification and cofermentation (SSCF) was carried out at approximately 15% total solids using conditioned dilute-acid pretreated yellow poplar feedstock, an adapted variant of National Renewable Energy Laboratory (NREL) xylose-fermenting Zymomonas mobilis and either commercial or N...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1999, Vol.77-79 (1-3), p.649-665
Main Authors: MCMILLAN, J. D, NEWMAN, M. M, TEMPLETON, D. W, MOHAGHEGHI, A
Format: Article
Language:English
Subjects:
Bacteria
Biofuel production
Biological and medical sciences
Biotechnology
Energy
Enzymes
Ethanol
Fermentation
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Studies
Trees
Zymomonas mobilis
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Summary:Simultaneous saccharification and cofermentation (SSCF) was carried out at approximately 15% total solids using conditioned dilute-acid pretreated yellow poplar feedstock, an adapted variant of National Renewable Energy Laboratory (NREL) xylose-fermenting Zymomonas mobilis and either commercial or NREL-produced cellulase enzyme preparations. In 7 d, at a cellulase loading of 12 filter paper units per gram cellulose (FPU/g), the integrated system produced more than 3% w/v ethanol and achieved 54% conversion of all potentially available biomass sugars (total sugars) entering SSCF. A control SSCF employing Sigmacell cellulose and a commercial cellulase at an enzyme loading of 14 FPU/g achieved 65% conversion of total sugars to ethanol.
ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/ABAB:79:1-3:649