Effects of dietary manganese on arterial glycosaminoglycan metabolism in Sprague-Dawley rats

The objectives of this study were to determine whether dietary manganese deficiency alters total glycosaminoglycan (GAG) concentration and composition and glycosyltransferase activity in rat aortas. Sprague-Dawley rats were fed either a manganese-deficient or a manganese-sufficient diet. Arterial GA...

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Bibliographic Details
Published in:Biological trace element research 1998-06, Vol.64 (1/3), p.275-288
Main Authors: Yang, P. (University of Maine, Orono, ME.), Klimis-Tavantzis, J
Format: Article
Language:English
Subjects:
Animals
AORTA
Aorta - drug effects
Aorta - metabolism
ARTERE
ARTERIAS
ARTERIES
Body Weight
CARBOHYDRATE METABOLISM
CARENCE EN SUBSTANCE NUTRITIVE
Cellulose acetate
Chromatography, High Pressure Liquid
DEFICIENCIAS NUTRITIVAS
Diet
Galactosyltransferases - metabolism
GLICOSAMINOGLICANOS
GLYCOSAMINOGLYCANE
GLYCOSAMINOGLYCANS
Glycosaminoglycans - metabolism
Heparan sulfate
Liver - drug effects
Liver - metabolism
Male
MANGANESE
Manganese - administration & dosage
Manganese - pharmacology
MANGANESO
Metabolism
METABOLISME DES GLUCIDES
METABOLISMO DE CARBOHIDRATOS
NUTRIENT DEFICIENCIES
Organ Size
RAT
RATA
RATS
Rats, Sprague-Dawley
Rodents
Spectrometry, Fluorescence
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Summary:The objectives of this study were to determine whether dietary manganese deficiency alters total glycosaminoglycan (GAG) concentration and composition and glycosyltransferase activity in rat aortas. Sprague-Dawley rats were fed either a manganese-deficient or a manganese-sufficient diet. Arterial GAGs were isolated and quantified by measuring uronic acid content. The individual GAGs were separated and quantified with cellulose acetate electrophoresis. The activity of the enzyme galactosyltransferase I was measured using a 100,000 g particulate fraction and 4-methylumbelliferylxyloside (Xyl-MU) as an acceptor. There was a significant decrease (p < or = 0.05) in uronic acid content in the manganese-deficient (1.18 +/- 0.08 mg/g) rat aortas as compared with the manganese-sufficient (1.59 +/- 0.10 mg/g) ones. Chondroitin sulfate and heparan sulfate concentrations were decreased by 38% (p < or = 0.01) and 36% (p < or = 0.05), respectively, in the manganese-deficient rat aortas. The incorporation of UDP-galactose to acceptors by the manganese-deficient rat aorta preparations was increased by 28% as compared to the manganese-sufficient preparations. These results indicate that manganese is involved in arterial GAG metabolism by affecting the enzyme galactosyltransferase and that changes in GAG concentration and composition with manganese deficiency may ultimately affect arterial wall integrity and subsequently cardiovascular health. This is the first work to demonstrate that manganese nutrition is important in arterial GAG metabolism.
ISSN:0163-4984
1559-0720
DOI:10.1007/BF02783343