Red Blood Cell Deformability Influences Platelets–Vessel Wall Interaction in Flowing Blood

Hematocrit and red cell size are important factors for the transport of blood platelets toward subendothelium in flowing blood. We report that red cell deformability also influences platelet transport. Red cell deformability was estimated with Couette-flow viscosimetry at a shear rate of 130 s–1 and...

Full description

Saved in:
Bibliographic Details
Published in:Blood 1984-12, Vol.64 (6), p.1228-1233
Main Authors: Aarts, Piet A.M.M., Heethaar, Robert M., Sixma, Jan J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood Platelets - physiology
Blood Vessels - physiology
Blood Viscosity
Chlorpromazine - pharmacology
Diamide - pharmacology
Erythrocyte Deformability - drug effects
Erythrocyte Indices
Fundamental and applied biological sciences. Psychology
Hematocrit
Humans
Isoxsuprine - pharmacology
Vertebrates: blood, hematopoietic organs, reticuloendothelial system
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hematocrit and red cell size are important factors for the transport of blood platelets toward subendothelium in flowing blood. We report that red cell deformability also influences platelet transport. Red cell deformability was estimated with Couette-flow viscosimetry at a shear rate of 130 s–1 and expressed as a 'T’ factor—a dimensionless parameter relating the relative viscosity and the hematocrit derived from the relationship: T = (1 — µ$$)/H, where µrel is the relative viscosity and H is the hematocrit. The normal value of T was within a narrow range (0.91 ± 0.02). Treatment of normal red cells with isoxsuprine and chlorpromazine caused decreased rigidity and decreased T. Cholesterol loading and treatment with diamide increased rigidity and increased T. In vitro perfusion experiments in an annular perfusion system with everted human umbilical arteries were performed with perfusates to which such treated red blood cells were added to investigate their influence on platelet adherence to artery subendothelium. Platelet adherence was well correlated with red cell rigidity, with increased adherence at increased rigidity and vice versa. A change in T of 0.10 corresponded to a change in platelet adherence of approximately 50%. These effects were more pronounced at a wall shear rate of 1,800 s–1 than at 300 s–1.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V64.6.1228.1228