Protein analysis of laser capture micro-dissected tissues revealed cell-type specific biological functions in developing barley grains

Both the nucellar projection (NP) and endosperm transfer cells (ETC) of the developing barley grain (harvested 8 days after flowering) were isolated by laser capture micro-dissection combined with pressure catapulting. Protein extracts were analyzed by nanoUPLC separation combined with ESI-Q-TOF mas...

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Bibliographic Details
Published in:Analytical & bioanalytical chemistry (Print) 2010-12, Vol.398 (7-8), p.2883-2893
Main Authors: Kaspar, Stephanie, Weier, Diana, Weschke, Winfriede, Mock, Hans-Peter, Matros, Andrea
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Barley
Biochemistry
Biological
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Developmental biology
Endosperm transfer cells
Enrichment
Exact sciences and technology
Food Science
Grains
Hordeum - chemistry
Hordeum - metabolism
Laboratory Medicine
Lasers
Micro-dissection
Microdissection - methods
Monitoring/Environmental Analysis
Multiplexed LC-MS
Nanostructure
Nucellar projection
Nutrients
Original Paper
Physiological aspects
Plant Proteins - analysis
Plant Proteins - isolation & purification
Proteins
seed development
Seeds - chemistry
Seeds - cytology
Seeds - metabolism
Spectrometric and optical methods
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
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Summary:Both the nucellar projection (NP) and endosperm transfer cells (ETC) of the developing barley grain (harvested 8 days after flowering) were isolated by laser capture micro-dissection combined with pressure catapulting. Protein extracts were analyzed by nanoUPLC separation combined with ESI-Q-TOF mass spectrometry. The majority of the ∼160 proteins identified were involved in translation, protein synthesis, or protein destination. The NP proteome was enriched for stress defense molecules, while proteins involved in assimilate transport and the mobilization of nutrients were common to both the NP and the ETC. The combined qualitative and quantitative protein profiling allowed for the identification of several proteins showing tissue specificity in their expression, which underlines the distinct biological functions of these two tissues within the developing barley grain.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-010-4120-y