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Transport of sugars or amino acids increases potassium efflux from isolated enterocytes
A technique to isolate epithelial cells from rabbit jejunum using hyaluronidase is described. The cells obtained retained their abilities to accumulate sugars and potassium ( 86Rb) against concentration gradients. Potassium efflux was monitored using cells preloaded with 86Rb and the rate constant o...
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| Published in: | FEBS letters 1983-11, Vol.163 (2), p.203-206 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4809-de18b923e8249983eb116676e846a3c81bc53aa083882c2819f555ee63385f7f3 |
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| cites | cdi_FETCH-LOGICAL-c4809-de18b923e8249983eb116676e846a3c81bc53aa083882c2819f555ee63385f7f3 |
| container_end_page | 206 |
| container_issue | 2 |
| container_start_page | 203 |
| container_title | FEBS letters |
| container_volume | 163 |
| creator | Brown, P.D. Burton, K.A. Sepúlveda, F.V. |
| description | A technique to isolate epithelial cells from rabbit jejunum using hyaluronidase is described. The cells obtained retained their abilities to accumulate sugars and potassium (
86Rb) against concentration gradients. Potassium efflux was monitored using cells preloaded with
86Rb and the rate constant of efflux was seen to increase when actively transported sugars or amino acids are added to the bathing medium.
The increase is related to the transport of the non-electrolyte, but not to volume regulatory events. |
| doi_str_mv | 10.1016/0014-5793(83)80819-9 |
| format | article |
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86Rb) against concentration gradients. Potassium efflux was monitored using cells preloaded with
86Rb and the rate constant of efflux was seen to increase when actively transported sugars or amino acids are added to the bathing medium.
The increase is related to the transport of the non-electrolyte, but not to volume regulatory events.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(83)80819-9</identifier><identifier>PMID: 6641937</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino acid transport ; amino acids ; Amino Acids - metabolism ; Amino Acids - pharmacology ; Animals ; Biological and medical sciences ; Biological Transport - drug effects ; Carbohydrate Metabolism ; Carbohydrates - pharmacology ; Cell physiology ; Enterocyte ; Female ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Intestinal Absorption - drug effects ; jejunum ; Jejunum - metabolism ; Male ; Membrane and intracellular transports ; Microvilli - metabolism ; Molecular and cellular biology ; potassium ; Potassium - metabolism ; Potassium transport ; Rabbit ; Rabbits ; Small intestine ; Sugar transport ; sugars</subject><ispartof>FEBS letters, 1983-11, Vol.163 (2), p.203-206</ispartof><rights>1983</rights><rights>FEBS Letters 163 (1983) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4809-de18b923e8249983eb116676e846a3c81bc53aa083882c2819f555ee63385f7f3</citedby><cites>FETCH-LOGICAL-c4809-de18b923e8249983eb116676e846a3c81bc53aa083882c2819f555ee63385f7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2F0014-5793%2883%2980819-9$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2F0014-5793%2883%2980819-9$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,1424,27957,27958,45609,45610</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9516402$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6641937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, P.D.</creatorcontrib><creatorcontrib>Burton, K.A.</creatorcontrib><creatorcontrib>Sepúlveda, F.V.</creatorcontrib><title>Transport of sugars or amino acids increases potassium efflux from isolated enterocytes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A technique to isolate epithelial cells from rabbit jejunum using hyaluronidase is described. The cells obtained retained their abilities to accumulate sugars and potassium (
86Rb) against concentration gradients. Potassium efflux was monitored using cells preloaded with
86Rb and the rate constant of efflux was seen to increase when actively transported sugars or amino acids are added to the bathing medium.
The increase is related to the transport of the non-electrolyte, but not to volume regulatory events.</description><subject>Amino acid transport</subject><subject>amino acids</subject><subject>Amino Acids - metabolism</subject><subject>Amino Acids - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Carbohydrate Metabolism</subject><subject>Carbohydrates - pharmacology</subject><subject>Cell physiology</subject><subject>Enterocyte</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Intestinal Absorption - drug effects</subject><subject>jejunum</subject><subject>Jejunum - metabolism</subject><subject>Male</subject><subject>Membrane and intracellular transports</subject><subject>Microvilli - metabolism</subject><subject>Molecular and cellular biology</subject><subject>potassium</subject><subject>Potassium - metabolism</subject><subject>Potassium transport</subject><subject>Rabbit</subject><subject>Rabbits</subject><subject>Small intestine</subject><subject>Sugar transport</subject><subject>sugars</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><recordid>eNqNkM-L1DAUgIMo6-zqf6CQg4h7qCZNm75cBF12VFjwsuIxZNIXibTNmNeq89-bMsMcVQiEvPe9H_kYeybFaymkfiOEbKq2M-oVqGsQIE1lHrCNhE5VqtHwkG3OyGN2SfRdlHfBLtiF1o00qtuwr_fZTbRPeeYpcFq-uUw8Ze7GOCXufOyJx8lndITE92l2RHEZOYYwLL95yGnkkdLgZuw5TjPm5A8z0hP2KLiB8OnpvmJftrf3Nx-ru88fPt28u6t8A8JUPUrYmVoh1I0xoHAnpdadRmi0Ux7kzrfKOQEKoPZ1WT60bYuolYI2dEFdsZfHvvucfixIsx0jeRwGN2FayILoWm0k_BOUytRG6K6AzRH0ORFlDHaf4-jywUphV_F2tWpXqxbKWcVbU8qen_ovuxH7c9HJdMm_OOUdeTeEot1HOmOmlboRdcG2R-xXHPDwX6Pt9vZ9vSbWeBG1Rtd93h4bYbH_M2K25CNOHvuY0c-2T_HvH_oDvQ6x_A</recordid><startdate>19831114</startdate><enddate>19831114</enddate><creator>Brown, P.D.</creator><creator>Burton, K.A.</creator><creator>Sepúlveda, F.V.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19831114</creationdate><title>Transport of sugars or amino acids increases potassium efflux from isolated enterocytes</title><author>Brown, P.D. ; Burton, K.A. ; Sepúlveda, F.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4809-de18b923e8249983eb116676e846a3c81bc53aa083882c2819f555ee63385f7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Amino acid transport</topic><topic>amino acids</topic><topic>Amino Acids - metabolism</topic><topic>Amino Acids - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Carbohydrate Metabolism</topic><topic>Carbohydrates - pharmacology</topic><topic>Cell physiology</topic><topic>Enterocyte</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Intestinal Absorption - drug effects</topic><topic>jejunum</topic><topic>Jejunum - metabolism</topic><topic>Male</topic><topic>Membrane and intracellular transports</topic><topic>Microvilli - metabolism</topic><topic>Molecular and cellular biology</topic><topic>potassium</topic><topic>Potassium - metabolism</topic><topic>Potassium transport</topic><topic>Rabbit</topic><topic>Rabbits</topic><topic>Small intestine</topic><topic>Sugar transport</topic><topic>sugars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, P.D.</creatorcontrib><creatorcontrib>Burton, K.A.</creatorcontrib><creatorcontrib>Sepúlveda, F.V.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, P.D.</au><au>Burton, K.A.</au><au>Sepúlveda, F.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transport of sugars or amino acids increases potassium efflux from isolated enterocytes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1983-11-14</date><risdate>1983</risdate><volume>163</volume><issue>2</issue><spage>203</spage><epage>206</epage><pages>203-206</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><abstract>A technique to isolate epithelial cells from rabbit jejunum using hyaluronidase is described. The cells obtained retained their abilities to accumulate sugars and potassium (
86Rb) against concentration gradients. Potassium efflux was monitored using cells preloaded with
86Rb and the rate constant of efflux was seen to increase when actively transported sugars or amino acids are added to the bathing medium.
The increase is related to the transport of the non-electrolyte, but not to volume regulatory events.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>6641937</pmid><doi>10.1016/0014-5793(83)80819-9</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1983-11, Vol.163 (2), p.203-206 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80756918 |
| source | Wiley Online Library |
| subjects | Amino acid transport amino acids Amino Acids - metabolism Amino Acids - pharmacology Animals Biological and medical sciences Biological Transport - drug effects Carbohydrate Metabolism Carbohydrates - pharmacology Cell physiology Enterocyte Female Fundamental and applied biological sciences. Psychology In Vitro Techniques Intestinal Absorption - drug effects jejunum Jejunum - metabolism Male Membrane and intracellular transports Microvilli - metabolism Molecular and cellular biology potassium Potassium - metabolism Potassium transport Rabbit Rabbits Small intestine Sugar transport sugars |
| title | Transport of sugars or amino acids increases potassium efflux from isolated enterocytes |
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