Transport of sugars or amino acids increases potassium efflux from isolated enterocytes

A technique to isolate epithelial cells from rabbit jejunum using hyaluronidase is described. The cells obtained retained their abilities to accumulate sugars and potassium ( 86Rb) against concentration gradients. Potassium efflux was monitored using cells preloaded with 86Rb and the rate constant o...

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Bibliographic Details
Published in:FEBS letters 1983-11, Vol.163 (2), p.203-206
Main Authors: Brown, P.D., Burton, K.A., Sepúlveda, F.V.
Format: Article
Language:English
Subjects:
Amino acid transport
amino acids
Amino Acids - metabolism
Amino Acids - pharmacology
Animals
Biological and medical sciences
Biological Transport - drug effects
Carbohydrate Metabolism
Carbohydrates - pharmacology
Cell physiology
Enterocyte
Female
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
Intestinal Absorption - drug effects
jejunum
Jejunum - metabolism
Male
Membrane and intracellular transports
Microvilli - metabolism
Molecular and cellular biology
potassium
Potassium - metabolism
Potassium transport
Rabbit
Rabbits
Small intestine
Sugar transport
sugars
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Summary:A technique to isolate epithelial cells from rabbit jejunum using hyaluronidase is described. The cells obtained retained their abilities to accumulate sugars and potassium ( 86Rb) against concentration gradients. Potassium efflux was monitored using cells preloaded with 86Rb and the rate constant of efflux was seen to increase when actively transported sugars or amino acids are added to the bathing medium. The increase is related to the transport of the non-electrolyte, but not to volume regulatory events.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(83)80819-9