Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis

Streptococcal pyrogenic exotoxin B is an extracellular cysteine protease. Only nephritis-associated strains of group A streptococci secrete this protease and this may be involved in the pathogenesis of post-streptococcal glomerulonephritis. Mice were actively immunized with a recombinant protease in...

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Bibliographic Details
Published in:Kidney international 2007-09, Vol.72 (6), p.716-724
Main Authors: Luo, Y.-H., Kuo, C.-F., Huang, K.-J., Wu, J.-J., Lei, H.-Y., Lin, M.T., Chuang, W.-J., Liu, C.-C., Lin, C.-F., Lin, Y.-S.
Format: Article
Language:English
Subjects:
Animals
anti-SPE B
Antibodies, Monoclonal - blood
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - pharmacology
Autoantibodies - blood
Autoantibodies - immunology
Autoantibodies - pharmacology
Bacterial diseases
Biological and medical sciences
Complement Activation - immunology
Cross Reactions
Cysteine Endopeptidases - immunology
Cysteine Endopeptidases - pharmacology
Disease Models, Animal
Endothelial Cells - cytology
Endothelial Cells - immunology
Glomerulonephritis
Glomerulonephritis - immunology
Glomerulonephritis - pathology
group A streptococcus
Human bacterial diseases
Humans
Immunization
Infectious diseases
Kidney Glomerulus - immunology
Kidney Glomerulus - pathology
Leukocytes - immunology
Medical sciences
Mice
Mice, Inbred BALB C
mouse model
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Proteinuria - immunology
Proteinuria - pathology
Recombinant Proteins - immunology
Recombinant Proteins - pharmacology
Staphylococcal infections, streptococcal infections, pneumococcal infections
Streptococcus
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Summary:Streptococcal pyrogenic exotoxin B is an extracellular cysteine protease. Only nephritis-associated strains of group A streptococci secrete this protease and this may be involved in the pathogenesis of post-streptococcal glomerulonephritis. Mice were actively immunized with a recombinant protease inactive exotoxin B mutant or passively immunized with exotoxin B antibody. Characteristics of glomerulonephritis were measured using histology, immunoglobulin deposition, complement activation, cell infiltration, and proteinuria. None of the mice given bovine serum albumin or exotoxin A as controls showed any marked changes. Immunoglobulin deposition, complement activation, and leukocyte infiltration occurred only in the glomeruli of exotoxin B-hyperimmunized mice. One particular anti-exotoxin B monoclonal antibody, 10G, was cross-reactive with kidney endothelial cells and it caused kidney injury and proteinuria when infused into mice. This cross-reactivity may be involved in the pathogenesis of glomerulonephritis following group A streptococcal infection.
ISSN:0085-2538
1523-1755
DOI:10.1038/sj.ki.5002407