Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein

The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is...

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Bibliographic Details
Published in:Journal of virological methods 2010-09, Vol.168 (1), p.98-102
Main Authors: SUNNA GUDMUNDSDOTTIR, Hólmfrídur, OLAFSDOTTIR, Katrín, RUT FRANZDOTTIR, Sigridur, ANDRESDOTTIR, Valgerdur
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
EGFP
Fundamental and applied biological sciences. Psychology
Genomic Instability
Green fluorescent protein
Green Fluorescent Proteins - biosynthesis
Green Fluorescent Proteins - genetics
Lentivirus
Maedi-visna virus
Maedi-visna virus (MVV)
Microbiology
Mutagenesis, Insertional
Pyrophosphatases - genetics
Recombination, Genetic
Serial Passage
Sheep
Staining and Labeling - methods
Techniques used in virology
Viral Proteins - genetics
Virology
Visna-maedi virus - genetics
Visna-maedi virus - pathogenicity
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Summary:The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2010.04.026