Adaptation of a high-voltage electron microscope for routine biological dark-field imaging

Relative merits of different objective aperture geometries for generating dark-field images in a high-voltage electron microscope (an AEI EM7) are considered. Using metal-bar beam stops in the objective lens diffraction plane, with large (500 μm diameter) limiting apertures, effective resolution in...

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Bibliographic Details
Published in:Ultramicroscopy 1983, Vol.11 (1), p.21-34
Main Authors: Mannella, Carmen A., Ratkowski, Anthony J.
Format: Article
Language:English
Subjects:
Animals
Brain - ultrastructure
Microscopy, Electron - instrumentation
Microscopy, Electron - methods
Rats
Staining and Labeling
T-Phages - ultrastructure
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Summary:Relative merits of different objective aperture geometries for generating dark-field images in a high-voltage electron microscope (an AEI EM7) are considered. Using metal-bar beam stops in the objective lens diffraction plane, with large (500 μm diameter) limiting apertures, effective resolution in the 2–4 nm range can be demonstrated for biological specimens.
ISSN:0304-3991
1879-2723
DOI:10.1016/0304-3991(83)90052-9