Simulated Blood Flow and the Effects on Prostacyclin Production in the Dog Femoral Artery

Effects of blood flow on the production of prostacyclin (PGI2) were examined in the canine femoral artery perfused ex vivo. Each artery was perfused in situ with medium 199, under conditions of simulated blood flow. To simulate the normal and abnormal blood flow waveforms at the same mean flow rate,...

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Bibliographic Details
Published in:Circulation research 1991-04, Vol.68 (4), p.1095-1099
Main Authors: Onohara, Toshihiro, Okadome, Kenichiro, Yamamura, Shinji, Mii, Shinsuke, Sugimachi, Keizo
Format: Article
Language:English
Subjects:
6-Ketoprostaglandin F1 alpha - analysis
Animals
Biological and medical sciences
Dogs
Epoprostenol - biosynthesis
Female
Femoral Artery - physiology
Fundamental and applied biological sciences. Psychology
Hemodynamics. Rheology
In Vitro Techniques
Male
Muscle, Smooth, Vascular - physiology
Muscles - blood supply
Perfusion - methods
Radioimmunoassay
Regional Blood Flow
Vertebrates: cardiovascular system
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Summary:Effects of blood flow on the production of prostacyclin (PGI2) were examined in the canine femoral artery perfused ex vivo. Each artery was perfused in situ with medium 199, under conditions of simulated blood flow. To simulate the normal and abnormal blood flow waveforms at the same mean flow rate, we developed a flow apparatus capable of making various waveforms by changing the duration when the electromagnetic valve in the circuit was open. Group I (n=7) was exposed to a steep acceleration waveform followed by a steep deceleration, as the normal flow waveform; group II (n=7) was exposed to a gentle sloping waveform in the deceleration phase, as the abnormal flow waveform. PGI2 was measured as 6-ketoprostaglandin F1α* PGI2 production was estimated as the cumulative production for the first 5 minutes (acute response) and as the production rate after the first 30 minutes (stable production rate). Under conditions of normal flow, the acute response was 5.87±2.16 ng/cm/5 min, whereas under conditions of abnormal flow, the rate was 2.20±0.27 ng/cm/5 min (p
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.68.4.1095