Biochemical and molecular characterization of three barley seed proteins with antifungal properties

We have purified three proteins from barley (Hordeum vulgare L.) seeds which synergistically inhibit the growth of fungi measured in a microtiter well assay. The proteins are a 26-kDa chitinase, a 30-kDa ribosome-inactivating protein, and a 32-kDa (1-3)-beta-glucanase. Full-length cDNAs encoding the...

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Bibliographic Details
Published in:The Journal of biological chemistry 1991-01, Vol.266 (3), p.1564-1573
Main Authors: LEAH, R, TOMMERUP, H, SVENDSEN, I, MUNDY, J
Format: Article
Language:English
Subjects:
(1-3)- beta -glucanase
Agronomy. Soil science and plant productions
Amino Acid Sequence
Antifungal Agents - isolation & purification
Base Sequence
beta-Glucosidase - genetics
beta-Glucosidase - isolation & purification
beta-Glucosidase - pharmacology
Biological and medical sciences
Blotting, Northern
Blotting, Southern
Chitinases - genetics
Chitinases - isolation & purification
Chitinases - pharmacology
Cloning, Molecular
DNA - genetics
Economic plant physiology
Enzymes
Fundamental and applied biological sciences. Psychology
Genes, Plant
Glucan 1,3-beta-Glucosidase
Hordeum - genetics
Hordeum vulgare
Molecular Sequence Data
N-Glycosyl Hydrolases
Nutrition. Photosynthesis. Respiration. Metabolism
Plant Proteins - genetics
Plant Proteins - isolation & purification
Plant Proteins - pharmacology
Ribosome Inactivating Proteins, Type 1
ribosome-inactivating protein
Ribosomes - drug effects
RNA, Messenger - genetics
Seeds - chemistry
Toxins, Biological
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Summary:We have purified three proteins from barley (Hordeum vulgare L.) seeds which synergistically inhibit the growth of fungi measured in a microtiter well assay. The proteins are a 26-kDa chitinase, a 30-kDa ribosome-inactivating protein, and a 32-kDa (1-3)-beta-glucanase. Full-length cDNAs encoding them were isolated and sequenced to determine the complete primary structures of the proteins. Northern hybridizations with the cDNAs as probes showed that the corresponding mRNAs accumulate differentially during seed development and germination. Chitinase mRNA accumulates to high levels in aleurone cells during late seed development and early germination, while high levels of mRNA encoding the ribosome-inactivating protein accumulate only in the starchy endosperm during late seed development. The glucanase mRNA accumulates to low levels during seed development and to higher levels in aleurone and seedling tissues during germination. Southern hybridizations showed that the three proteins are encoded by small families of three to eight genes. Their biological roles and potential use in genetic engineering studies are discussed.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)52331-0