Immunochemical Characterization of the Mucoid Exopolysaccharide of Pseudomonas aeruginosa

Alginic acid-like mucoid exopolysaccharide was isolated from three strains of Pseudomonas aeruginosa obtained from the sputa of patients with cystic fibrosis. Purified mucoid antigens were >99% uronic acid. With a hemagglutination assay, antibody responses to the mucoid exopolysaccharide were doc...

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Bibliographic Details
Published in:The Journal of infectious diseases 1983-03, Vol.147 (3), p.494-503
Main Authors: Pier, Gerald B., Matthews, Wallace J., Eardley, Diane D.
Format: Article
Language:English
Subjects:
Adult
Alginates
Animals
Antibodies
Antigens
Antigens, Bacterial - immunology
Biochemistry
Child
Cystic fibrosis
Cystic Fibrosis - immunology
Epitopes
Glycosaminoglycans - immunology
Glycosaminoglycans - isolation & purification
Hemagglutination Inhibition Tests
Hemagglutination Tests
Humans
Immune Sera - immunology
Lipopolysaccharides
Microbiology and Laboratory Diagnosis
Molecular Weight
Polysaccharides
Polysaccharides, Bacterial - immunology
Polysaccharides, Bacterial - isolation & purification
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Pseudomonas aeruginosa - metabolism
Rabbits - immunology
Uronic acids
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Summary:Alginic acid-like mucoid exopolysaccharide was isolated from three strains of Pseudomonas aeruginosa obtained from the sputa of patients with cystic fibrosis. Purified mucoid antigens were >99% uronic acid. With a hemagglutination assay, antibody responses to the mucoid exopolysaccharide were documented after immunization of rabbits with either whole mucoid organisms or purified mucoid exopolysaccharide. The mucoid antigen from one strain (no. 2192) was composed predominantly of a single serologic epitope shared among 40 alginate exopolysaccharides from different clinical isolates. The mucoid exopolysaccharide from the other two strains (nos. 1 and 258) had a serotype-specific determinant in addition to the common epitope. Analyses of antibody in sera from normal adults, children, and patients with cystic fibrosis culturepositive and culture-negative for mucoid P. aeruginosa showed a highly significant (P
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/147.3.494