Evidence for a resident subset of cells with SP phenotype in the C2C12 myogenic line: a tool to explore muscle stem cell biology

Muscle satellite cells are heterogeneous and present functional disparities, some of them behaving as multipotent stem cells. Yet their phenotype is obscure and their isolation remains elusive. The ability to purify stem cells from a wide variety of tissues by using Hoechst 33342 staining/FACS metho...

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Bibliographic Details
Published in:Experimental cell research 2004-03, Vol.294 (1), p.254-268
Main Authors: Benchaouir, Rachid, Rameau, Philippe, Decraene, Charles, Dreyfus, Patrick, Israeli, David, Piétu, Geneviève, Danos, Olivier, Garcia, Luis
Format: Article
Language:English
Subjects:
Animals
ATP Binding Cassette Transporter, Sub-Family B - metabolism
ATP-Binding Cassette Transporters - metabolism
Benzimidazoles
C2C12
Cell Cycle
Cell Differentiation
Cell Line
Cells, Cultured
Fluorescent Dyes
Gene Expression Profiling
mdr1
Mice
Mice, Inbred C57BL
Muscle
Myoblasts, Skeletal - cytology
Myoblasts, Skeletal - metabolism
Myoblasts, Skeletal - ultrastructure
Phenotype
Satellite Cells, Skeletal Muscle - cytology
Satellite Cells, Skeletal Muscle - metabolism
Satellite Cells, Skeletal Muscle - ultrastructure
SP phenotype
Stem cell
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Summary:Muscle satellite cells are heterogeneous and present functional disparities, some of them behaving as multipotent stem cells. Yet their phenotype is obscure and their isolation remains elusive. The ability to purify stem cells from a wide variety of tissues by using Hoechst 33342 staining/FACS methods has permitted access to this category of cells (side population, or SP) in a manner independent of antibodies. Here, we show that the C2C12 myogenic line comprises a minor population of cells with SP phenotype. These cells are growth-arrested and delayed in their ability to differentiate. Dye efflux in C2C12-derived SPs is likely mediated by mdr1a, whose overexpression results in increased dedifferentiation. Interestingly, growth-arrested SPs rapidly appear in purified MP populations, thus suggesting a dynamic equilibrium among different states of differentiation. Finally, transcriptional profiling of C2C12-derived SP and MP cells corroborates the many similarities of SP to stem cells.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2003.11.005