Transplantation‐Induced Functional/Morphological Changes in Rat Aorta Allografts Differ from Those in Arteries of Rat Kidney Allografts

The functional/morphological changes observed in rat aorta allografts were compared with those seen in the arteries of rat kidney allografts. Untreated allografts (F344‐to‐LEW) were collected at various times post‐transplantation (Tx). Vascular smooth muscle cell (SMC) constriction to phenylephrine...

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Bibliographic Details
Published in:American journal of transplantation 2004-02, Vol.4 (2), p.188-195
Main Authors: Andriambeloson, Emile, Cannet, Catherine, Pally, Charles, Klanke, Bernd, Bruns, Christian, Zerwes and, Hans‐Günter, Bigaud, Marc
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Animals
Aorta - pathology
Aorta - transplantation
Aorta allograft
chronic graft arteriosclerosis
Endothelium, Vascular - drug effects
Endothelium, Vascular - physiology
Endothelium, Vascular - physiopathology
Glomerular Filtration Rate
Graft Survival - physiology
kidney allograft
Kidney Transplantation - pathology
Kidney Transplantation - physiology
Phenylephrine - pharmacology
rat
Rats
Rats, Inbred F344
Rats, Inbred Lew
Renal Artery - drug effects
Renal Artery - pathology
Renal Artery - physiology
Renal Artery - transplantation
Time Factors
Transplantation, Homologous - pathology
Transplantation, Homologous - physiology
vascular function
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Summary:The functional/morphological changes observed in rat aorta allografts were compared with those seen in the arteries of rat kidney allografts. Untreated allografts (F344‐to‐LEW) were collected at various times post‐transplantation (Tx). Vascular smooth muscle cell (SMC) constriction to phenylephrine (Phe) and endothelial cell (EC)‐dependent relaxation to acetylcholine (Ach) were assessed. Neointima formation in graft vessels was assessed by histology. In aorta allografts, the effects of Phe and Ach were irreversibly abolished within 3–2 weeks post‐Tx. Neointima formation was consistently detected between 4 and 8 weeks post‐Tx. In kidney allografts, sign of vasculopathy was seen in 10, 30 and 40% of resistance arteries at 8, 16 and 33 weeks post‐Tx, respectively. In the main renal artery, substantial neointima formation was not apparent before 33 weeks post‐Tx, the vasoconstrictor effect of Phe was fully maintained until then, and Ach‐induced vasorelaxation was irreversibly reduced by approximately 70% from week 2 post‐Tx onwards. These results indicate that the post‐Tx functional/morphological changes seen in aorta allografts do not reflect those seen in arteries of kidney allografts. Hence, renal arteries from rat kidney allografts can be considered as a more relevant model to study the cascade of events leading to Tx‐induced CGA in solid organ allografts.
ISSN:1600-6135
1600-6143
DOI:10.1046/j.1600-6143.2003.00319.x