Characterization of two genes expressed in the salivary glands of the Hessian fly, Mayetiola destructor (Say)

Two genes, SSGP-11A1 and SSGP-12A1, have been isolated that encodes proteins with a secretion signal peptide at theN-terminals from the Hessian fly ( Mayetiola destructor (Say)). The SSGP-11A1 gene contains one small intron (89 bp) and encodes a putative protein with 79 amino acids. The first 18 ami...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2004-03, Vol.34 (3), p.229-237
Main Authors: Liu, Xuming, Fellers, John P, Wilde, Gerald E, Stuart, Jeffrey J, Chen, Ming-Shun
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Animals
Base Sequence
Cecidomyiidae
chromosomes
complementary DNA
Diptera - genetics
Diptera - metabolism
DNA, Complementary - genetics
Gene Expression
gene location
genes
Genes, Insect
Hessian fly
In Situ Hybridization, Fluorescence
insect pests
Insect Proteins - genetics
Larva - metabolism
larvae
Mayetiola destructor
messenger RNA
Molecular Sequence Data
nucleotide sequences
protein secretion
Protein Sorting Signals - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Salivary gland
salivary glands
Salivary Glands - metabolism
Secreted protein
secretion signal peptide
sequence alignment
Sequence Homology, Amino Acid
signal peptide
SSGP-11A1 gene
SSGP-12A1 gene
Tissue Distribution
Wheat
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Summary:Two genes, SSGP-11A1 and SSGP-12A1, have been isolated that encodes proteins with a secretion signal peptide at theN-terminals from the Hessian fly ( Mayetiola destructor (Say)). The SSGP-11A1 gene contains one small intron (89 bp) and encodes a putative protein with 79 amino acids. The first 18 amino acids constitute a putative secretion signal peptide. The SSGP-12A1 gene contains three small introns and encodes a putative protein with 234 amino acids. The first 19 amino acids constitute a putative secretion signal peptide. Northern blot analysis revealed that both of the genes are primarily expressed in the salivary glands of Hessian fly larvae, the feeding stage of the insect. These observations are consistent with the possibility that the proteins encoded by them are secreted into host plants during feeding. Even though both genes are exclusively expressed in Hessian fly larvae, the expression profiles between them were quite different in insects at different instars. The SSGP-11A1 gene was expressed in all instars of larvae while the SSGP-12A1 gene was almost exclusively expressed in the first instar larvae. The differential expression suggests that the proteins encoded by these two genes may perform different functions. In situ hybridization revealed that the SSGP-11A1 gene is located on the short arm of chromosome A1 while SSGP-12A1 gene is on the long arm of chromosome A2.
ISSN:0965-1748
1879-0240
DOI:10.1016/j.ibmb.2003.10.008