Further characterization of 'sweet potato virus 2': a distinct species of the genus Potyvirus

An incompletely described potyvirus isolate from sweet potato in Taiwan, referred to as 'sweet potato virus 2' (SPV2), was further characterised. Electron microscopy revealed that SPV2 has filamentous particles of 850 nm in length and induces cytoplasmic cylindrical inclusions consisting o...

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Bibliographic Details
Published in:Archives of virology 2004-02, Vol.149 (2), p.225-239
Main Authors: ATEKA, E. M, BARG, E, NJERU, R. W, LESEMANN, D.-E, VETTEN, H. J
Format: Article
Language:English
Subjects:
ACP gene
Amino Acid Sequence
ANIb gene
Animals
Biological and medical sciences
Crop diseases
Cytopathogenic Effect, Viral
Fundamental and applied biological sciences. Psychology
Human viral diseases
Infectious diseases
Insect Vectors
Ipomoea batatas - virology
Medical sciences
Microbiology
Miscellaneous
Molecular Sequence Data
Phylogeny
Plant Diseases - virology
Plants - virology
Potatoes
Potyvirus - classification
Potyvirus - genetics
Potyvirus - physiology
Potyvirus - ultrastructure
Proteins
Sequence Analysis, RNA
Serology
Species Specificity
sweet potato virus 2
sweet potato virus Y
Taiwan
Viral diseases
Viral Structural Proteins - chemistry
Viral Structural Proteins - genetics
Virology
Viruses
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Description
Summary:An incompletely described potyvirus isolate from sweet potato in Taiwan, referred to as 'sweet potato virus 2' (SPV2), was further characterised. Electron microscopy revealed that SPV2 has filamentous particles of 850 nm in length and induces cytoplasmic cylindrical inclusions consisting of pinwheels and scrolls. The virus was mechanically transmitted to several species of the genera Chenopodium, Datura, Nicotiana, and Ipomoea. Two biotypes of Myzus persicae transmitted SPV2 in a non-persistent manner. Decoration titer experiments revealed a distant serological relationship between SPV2 and other potyviruses infecting sweet potato. The 3'-terminal 2006 nucleotides of the viral RNA were determined and shown to be a potyviral genome fragment comprising the coding region for the C-terminal half of the NIb protein, the entire coat protein cistron, and the 3' untranslated region (UTR). Comparison of the capsid protein and 3' UTR sequences of SPV2 with those of other potyviruses demonstrated that it is a distinct member of the genus Potyvirus (family Potyviridae). We propose that SPV2 is named Sweet potato virus Y.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-003-0233-3