Oocyte regulation of anti-Müllerian hormone expression in granulosa cells during ovarian follicle development in mice

In the ovarian follicle, anti-Müllerian hormone ( Amh) mRNA is expressed in granulosa cells from primary to preovulatory stages but becomes restricted to cumulus cells following antrum formation. Anti-Müllerian hormone regulates follicle development by attenuating the effects of follicle stimulating...

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Bibliographic Details
Published in:Developmental biology 2004-02, Vol.266 (1), p.201-208
Main Authors: Salmon, Nicholas A, Handyside, Alan H, Joyce, Ieuan M
Format: Article
Language:English
Subjects:
Animals
Anti-Mullerian Hormone
Anti-Müllerian hormone
Base Sequence
DNA Primers
Female
Follicle
Glycoproteins - genetics
Granulosa cells
Granulosa Cells - metabolism
Mice
Mouse
Oocyte
Oocytes - physiology
Ovary
RNA, Messenger - genetics
Testicular Hormones - genetics
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Summary:In the ovarian follicle, anti-Müllerian hormone ( Amh) mRNA is expressed in granulosa cells from primary to preovulatory stages but becomes restricted to cumulus cells following antrum formation. Anti-Müllerian hormone regulates follicle development by attenuating the effects of follicle stimulating hormone on follicle growth and inhibiting primordial follicle recruitment. To examine the role of the oocyte in regulating granulosa cell Amh expression in the mouse, isolated oocytes and granulosa cells were co-cultured and Amh mRNA levels were analysed by real-time RT-PCR. Expression in freshly isolated granulosa cells increased with preantral follicle development but was low in the cumulus and virtually absent in the mural granulosa cells of preovulatory follicles. When preantral granulosa cells were co-cultured with oocytes from early preantral, late preantral or preovulatory follicles, and when oocytes from preovulatory follicles were co-cultured with cumulus granulosa cells, Amh expression was increased at least 2-fold compared with granulosa cells cultured alone. With oocytes from preantral but not preovulatory follicles, this was a short-range effect only observed with granulosa cells in close apposition to oocytes. We conclude that stage-specific oocyte regulation of Amh expression may play a role in intra- and inter-follicular coordination of follicle development.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2003.10.009