Reagentless Detection and Classification of Individual Bioaerosol Particles in Seconds

The rapid chemical analysis of individual cells is an analytical capability that will profoundly impact many fields including bioaerosol detection for biodefense and cellular diagnostics for clinical medicine. This article describes a mass spectrometry-based analytical technique for the real-time an...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2004-01, Vol.76 (2), p.373-378
Main Authors: Fergenson, David P, Pitesky, Maurice E, Tobias, Herbert J, Steele, Paul T, Czerwieniec, Gregg A, Russell, Scott C, Lebrilla, Carlito B, Horn, Joanne M, Coffee, Keith R, Srivastava, Abneesh, Pillai, Segaran P, Shih, Meng-Ta Peter, Hall, Howard L, Ramponi, Albert J, Chang, John T, Langlois, Richard G, Estacio, Pedro L, Hadley, Robert T, Frank, Matthias, Gard, Eric E
Format: Article
Language:English
Subjects:
Aerosols
Aerosols - analysis
Air Microbiology
Analytical chemistry
Atoms & subatomic particles
Bacillus - chemistry
Bacillus - isolation & purification
Bacillus atrophaeus
Bacillus thuringiensis
Bacillus thuringiensis - chemistry
Bacillus thuringiensis - isolation & purification
Biochemistry
Chemistry
Classification
Clostridium - chemistry
Clostridium - isolation & purification
Complex Mixtures - analysis
Culture Media - pharmacology
Exact sciences and technology
Mass Spectrometry - methods
Reproducibility of Results
Spectrometric and optical methods
Spores, Bacterial - classification
Spores, Bacterial - drug effects
Spores, Bacterial - isolation & purification
Spores, Fungal - chemistry
Spores, Fungal - classification
Spores, Fungal - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The rapid chemical analysis of individual cells is an analytical capability that will profoundly impact many fields including bioaerosol detection for biodefense and cellular diagnostics for clinical medicine. This article describes a mass spectrometry-based analytical technique for the real-time and reagentless characterization of individual airborne cells without sample preparation. We characterize the mass spectral signature of individual Bacillus spores and demonstrate the ability to distinguish two Bacillus spore species, B. thuringiensis and B. atrophaeus, from one another very accurately and from the other biological and nonbiological background materials tested with no false positives at a sensitivity of 92%. This example demonstrates that the chemical differences between these two Bacillus spore species are consistently and easily detected within single cells in seconds.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac034467e