Protection against wild-type murine gammaherpesvirus-68 latency by a latency-deficient mutant

Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK Correspondence Philip Stevenson pgs27{at}mole.bio.cam.ac.uk A murine gammaherpesvirus-68 (MHV-68) mutant with deregulated transcription of its ORF50 transactivator was severely impaired i...

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Bibliographic Details
Published in:Journal of general virology 2004-01, Vol.85 (1), p.131-135
Main Authors: Boname, Jessica M, Coleman, Heather M, May, Janet S, Stevenson, Philip G
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Antibodies, Viral - blood
Biological and medical sciences
DNA, Viral - analysis
DNA, Viral - isolation & purification
Fundamental and applied biological sciences. Psychology
Gammaherpesvirinae - genetics
Gammaherpesvirinae - immunology
Gammaherpesvirinae - pathogenicity
Gene Expression Regulation, Viral
Herpesviridae Infections - immunology
Herpesviridae Infections - prevention & control
Immunity, Cellular
Lung - virology
Mice
Mice, Inbred C57BL
Microbiology
Miscellaneous
Murine gammaherpesvirus 68
Mutation
Spleen - virology
Vaccination
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Virology
Virus Latency - genetics
Virus Latency - physiology
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Summary:Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK Correspondence Philip Stevenson pgs27{at}mole.bio.cam.ac.uk A murine gammaherpesvirus-68 (MHV-68) mutant with deregulated transcription of its ORF50 transactivator was severely impaired in latency establishment. The deregulated virus showed reduced immunogenicity, probably reflecting a lower antigen load. However, it still elicited effective immunity to a subsequent wild-type (WT) virus challenge. Infection was not completely prevented, but was very substantially reduced in extent and the long-term level of WT viral DNA in lungs and spleens remained low. Thus latency-deficient MHV-68 illustrates a possible general approach to creating attenuated gammaherpesvirus vaccines that can protect against pathogenic WT infections.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.19592-0