Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells

We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective a...

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Bibliographic Details
Published in:Nature biotechnology 2004-01, Vol.22 (1), p.53-54
Main Authors: Andrews, Peter W, Draper, Jonathan S, Smith, Kath, Gokhale, Paul, Moore, Harry D, Maltby, Edna, Johnson, Julie, Meisner, Lorraine, Zwaka, Thomas P, Thomson, James A
Format: Article
Language:English
Subjects:
Aneuploidy
Animals
Biological and medical sciences
Biotechnology
Cell Culture Techniques - methods
Cell Differentiation
Cells, Cultured
chromosome 17
Chromosome Aberrations
Chromosome Banding
Chromosomes
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 17
Embryo, Mammalian - cytology
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Humans
In Situ Hybridization, Fluorescence
Industrial applications and implications. Economical aspects
Karyotyping
Laboratories
Miscellaneous
Models, Genetic
Specimen Handling
Stem cells
Stem Cells - cytology
Time Factors
Tumors
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Summary:We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt922