Alterations of Na,K‐ATPase Isoenzymes in the Rat Diabetic Neuropathy: Protective Effect of Dietary Supplementation with n‐3 Fatty Acids

: Diabetic neuropathy is a degenerative complication of diabetes accompanied by an alteration of nerve conduction velocity (NCV) and Na,K‐ATPase activity. The present study in rats was designed first to measure diabetes‐induced abnormalities in Na,K‐ATPase activity, isoenzyme expression, fatty acid...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neurochemistry 1998-08, Vol.71 (2), p.732-740
Main Authors: Gerbi, A., Maixent, J.‐M., Barbey, O., Jamme, I., Pierlovisi, M., Coste, T., Pieroni, G., Nouvelot, A., Vague, P., Raccah, D.
Format: Article
Language:English
Subjects:
Animals
Arachidonic Acid - metabolism
Associated diseases and complications
Biological and medical sciences
Cell Membrane - enzymology
Diabetes. Impaired glucose tolerance
Diabetic Neuropathies - diet therapy
Diabetic Neuropathies - enzymology
Dietary Fats, Unsaturated - pharmacology
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Enzyme Inhibitors - pharmacology
Fatty acids
Fatty Acids, Omega-3 - pharmacology
Fish oil
Fish Oils - pharmacology
Isoenzyme
Isoenzymes - antagonists & inhibitors
Isoenzymes - metabolism
Male
Medical sciences
Na,K‐ATPase
Neuropathy
Olive Oil
Ouabain - pharmacology
Plant Oils - pharmacology
Rats
Rats, Sprague-Dawley
Sciatic Nerve - drug effects
Sciatic Nerve - metabolism
Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors
Sodium-Potassium-Exchanging ATPase - metabolism
Subcellular Fractions - enzymology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:: Diabetic neuropathy is a degenerative complication of diabetes accompanied by an alteration of nerve conduction velocity (NCV) and Na,K‐ATPase activity. The present study in rats was designed first to measure diabetes‐induced abnormalities in Na,K‐ATPase activity, isoenzyme expression, fatty acid content in sciatic nerve membranes, and NCV and second to assess the preventive ability of a fish oil‐rich diet (rich in n‐3 fatty acids) on these abnormalities. Diabetes was induced by intravenous streptozotocin injection. Diabetic animals (D) and nondiabetic control animals (C) were fed the standard rat chow either without supplementation or supplemented with either fish oil (DM, CM) or olive oil (DO, CO) at a daily dose of 0.5 g/kg by gavage during 8 weeks. Analysis of the fatty acid composition of purified sciatic nerve membranes from diabetic animals showed a decreased incorporation of C16:1(n‐7) fatty acids and arachidonic acids. Fish oil supplementation changed the fatty acid content of sciatic nerve membranes, decreasing C18:2(n‐6) fatty acids and preventing the decreases of arachidonic acids and C18:1(n‐9) fatty acids. Protein expression of Na,K‐ATPase α subunits, Na,K‐ATPase activity, and ouabain affinity were assayed in purified sciatic nerve membranes from CO, DO, and DM. Na,K‐ATPase activity was significantly lower in sciatic nerve membranes of diabetic rats and significantly restored in diabetic animals that received fish oil supplementation. Diabetes induced a specific decrease of α1‐ and α3‐isoform activity and protein expression in sciatic nerve membranes. Fish oil supplementation restored partial activity and expression to varying degrees depending on the isoenzyme. These effects were associated with a significant beneficial effect on NCV. This study indicates that fish oil has beneficial effects on diabetes‐induced alterations in sciatic nerve Na,K‐ATPase activity and function.
ISSN:0022-3042
1471-4159
DOI:10.1046/j.1471-4159.1998.71020732.x