A yeast glutamine tRNA signals nitrogen status for regulation of dimorphic growth and sporulation

Dimorphic growth of the budding yeast Saccharomyces cerevisiae is regulated by the quality of the nitrogen supply. On a preferred nitrogen source diploid cells grow as ellipsoidal cells by using a bipolar pattern of budding, whereas on a poor nitrogen source a unipolar pattern of budding is adopted,...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-07, Vol.95 (15), p.8619-8624
Main Authors: Murray, L.E, Rowley, N, Dawes, I.W, Johnston, G.C, Singer, R.A
Format: Article
Language:English
Subjects:
Anticodon
ARN DE TRANSFERENCIA
ARN DE TRANSFERT
AZOTE
Base Sequence
Biological Sciences
BIPOLAR CELL BUDDING
Calcium-Calmodulin-Dependent Protein Kinases - metabolism
CAR1 GENE
CELL DIVISION
Cell growth
Cells
Cellular biology
Codon
CODONS
Diploidy
DISPONIBILIDAD DE NUTRIENTES
DISPONIBILITE D'ELEMENT NUTRITIF
DIVISION CELLULAIRE
DIVISION CELULAR
ESPORULACION
EXPRESION GENICA
EXPRESSION DES GENES
FUNGAL MORPHOLOGY
Fungal Proteins - metabolism
GENE
GENE EXPRESSION
GENES
GLUTAMINA
GLUTAMINE
MEIOSE
MEIOSIS
Messenger RNA
MORFOLOGIA FUNGICA
MORPHOLOGIE DE CHAMPIGNON
MUTACION
MUTATION
NITROGEN
Nitrogen - metabolism
NITROGENO
Nucleic Acid Conformation
NUCLEOTIDE SEQUENCE
NUTRIENT AVAILABILITY
Plasmids
Protein Biosynthesis
Proteins
PSEUDOHYPHAE
Ribonucleic acid
RNA
RNA, Transfer, Gln - chemistry
RNA, Transfer, Gln - genetics
RNA, Transfer, Gln - metabolism
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Signal Transduction
Spores, Fungal
SPORULATION
TRANSCRIPCION
TRANSCRIPTION
Transcription Factors - metabolism
TRANSFER RNA
TRANSLATION
UNIPOLAR CELL BUDDING
Yeast
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Summary:Dimorphic growth of the budding yeast Saccharomyces cerevisiae is regulated by the quality of the nitrogen supply. On a preferred nitrogen source diploid cells grow as ellipsoidal cells by using a bipolar pattern of budding, whereas on a poor nitrogen source a unipolar pattern of budding is adopted, resulting in extended pseudohyphal chains of filamentous cells. Here we report that the quality of the nitrogen source is signaled by the glutamine tRNA isoform with a 5'-CUG anticodon (tRNA(CUG)). Mutations that alter this tRNA impair assessment of the nitrogen supply without measurably affecting protein synthesis, so that mutant cells display pseudohyphal growth even on a preferred nitrogen source. The nitrogen status for other nitrogen-responsive processes such as catabolic gene expression and sporulation also is signaled by this tRNA: mutant cells inappropriately induce he nitrogen-repressed gene CAR1 and undergo precocious sporulation in nitrogen-rich media. Therefore, in addition to its role in mRNA translation, this tRNA also transduces nitrogen signals that regulate development
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.15.8619