Evidence for incomplete replication of a penguin poxvirus in cells of mammalian origin

LM Stannard, D Marais, D Kow and KR Dumbell Department of Medical Microbiology, University of Cape Town Medical School, South Africa. stannard@medmicro.uct.ac.za The recent discovery of a novel poxvirus [penguin-pox virus (PPV)] from Jackass penguins offers the potential of a unique candidate vaccin...

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Bibliographic Details
Published in:Journal of general virology 1998-07, Vol.79 (7), p.1637-1646
Main Authors: Stannard, LM, Marais, D, Kow, D, Dumbell, KR
Format: Article
Language:English
Subjects:
Animals
Birds - virology
Cattle
Cell Line
Cercopithecus aethiops
Chick Embryo
Cytoplasm - virology
DNA, Viral
Gene Expression
HeLa Cells
Humans
Mammals
Microscopy, Electron
Poxviridae - genetics
Poxviridae - physiology
Poxviridae - ultrastructure
Rabbits
Vero Cells
Virus Replication
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Summary:LM Stannard, D Marais, D Kow and KR Dumbell Department of Medical Microbiology, University of Cape Town Medical School, South Africa. stannard@medmicro.uct.ac.za The recent discovery of a novel poxvirus [penguin-pox virus (PPV)] from Jackass penguins offers the potential of a unique candidate vaccine vector for use in mammals. Infectivity studies were therefore undertaken using a number of mammalian cell lines and chick embryo fibroblasts (CEF). It was shown that the simian CV-1 cell line was able to support replication of the PPV DNA, but no infectious progeny virus could be recovered from the infected cells. Electron microscopy was used to establish the extent of virus morphogenesis in CV-1 cells as compared to that in both chorio-allantoic membranes (CAMs) of hens' eggs and CEF cells. It appears that CV-1 cells are able to support partial maturation of PPV, but that morphogenesis does not proceed to the stage of mature infectious particles. Vaccinia virus promoters were successful in achieving transient gene expression in PPV-infected cells.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-79-7-1637