Prospective Study for the Detection of Anaplasma marginale Theiler, 1911 (Rickettsiales: Anaplasmataceae) in Costa Rica

A prospective study was conducted to assess the dynamics of the infection and host response to Anaplasma marginale in one closed herd in the dry tropical forest of Costa Rica. The study subjects were the dams and their calves born during 1 breeding season (1995-1996). All cows were sampled at 3 mont...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1998-06, Vol.849 (1), p.226-233
Main Authors: HERRERO, M. V., PEREZ, E., GOFF, W. L., DE ECHAIDE, S. TORIONI, KNOWLES, D. P., McELWAIN, T. F., ALVAREZ, V., ALVAREZ, A., BUENING, G. M.
Format: Article
Language:English
Subjects:
Anaplasma - isolation & purification
Anaplasma marginale
Anaplasmosis - diagnosis
Anaplasmosis - epidemiology
Animals
Antibodies, Bacterial - blood
Carrier State - diagnosis
Cattle
Cohort Studies
Costa Rica
Enzyme-Linked Immunosorbent Assay
Female
Incidence
Ixodidae
Polymerase Chain Reaction
Reproducibility of Results
Seasons
Tick Infestations - complications
Tick Infestations - veterinary
Tropical Climate
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Summary:A prospective study was conducted to assess the dynamics of the infection and host response to Anaplasma marginale in one closed herd in the dry tropical forest of Costa Rica. The study subjects were the dams and their calves born during 1 breeding season (1995-1996). All cows were sampled at 3 month intervals for antibody detection using a competitive ELISA (cELISA) and for antigen detection using PCR/nonradioactive probe assay. All 24 calves born during the study were individually identified at birth and subsequently sampled each month for PCR and cELISA. Ticks were identified from all animals throughout the entire study period. The results from this study confirmed that the cELISA is a reliable assay for identifying new and carrier infections and that carrier infections can exist at levels below that detectable by PCR. In addition, it was demonstrated that calves born in this region will most likely be exposed to Anaplasma within the first 6 months of age.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1998.tb11053.x