C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic archaea

Methanogenic and sulfate-reducing Archaea are considered to have an energy metabolism involving C1 transfer coenzymes and enzymes unique for this group of strictly anaerobic microorganisms. An aerobic methylotrophic bacterium, Methylobacterium extorquens AM1, was found to contain a cluster of genes...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1998-07, Vol.281 (5373), p.99-102
Main Authors: CHISTOSERDOVA, L, VORHOLT, J. A, THAUER, R. K, LIDSTROM, M. E
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Aminohydrolases - chemistry
Aminohydrolases - genetics
Aminohydrolases - isolation & purification
Aminohydrolases - metabolism
Bacteria
Bacteriology
Biological and medical sciences
Biological Evolution
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Enzymes
Escherichia coli - enzymology
Escherichia coli - genetics
Euryarchaeota - enzymology
Euryarchaeota - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Archaeal
Genes, Bacterial
Genetic aspects
Genetics
Gram-Negative Aerobic Rods and Cocci - enzymology
Gram-Negative Aerobic Rods and Cocci - genetics
Hydroxymethyl and Formyl Transferases - chemistry
Hydroxymethyl and Formyl Transferases - genetics
Hydroxymethyl and Formyl Transferases - isolation & purification
Hydroxymethyl and Formyl Transferases - metabolism
Metabolism
Methanobacteriaceae
Methanogens
Methanol - metabolism
Microbial metabolism
Microbiology
Mission oriented research
Molecular Sequence Data
Mutation
NAD - metabolism
NADP - metabolism
Oxidation-Reduction
Pterins - chemistry
Pterins - isolation & purification
Pterins - metabolism
Sequence Alignment
Space life sciences
Succinic Acid - metabolism
Transformation, Bacterial
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Summary:Methanogenic and sulfate-reducing Archaea are considered to have an energy metabolism involving C1 transfer coenzymes and enzymes unique for this group of strictly anaerobic microorganisms. An aerobic methylotrophic bacterium, Methylobacterium extorquens AM1, was found to contain a cluster of genes that are predicted to encode some of these enzymes and was shown to contain two of the enzyme activities and one of the methanogenic coenzymes. Insertion mutants were all unable to grow on C1 compounds, suggesting that the archaeal enzymes function in aerobic C1 metabolism. Thus, methylotrophy and methanogenesis involve common genes that cross the bacterial/archaeal boundaries.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.281.5373.99